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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-6-23
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pubmed:abstractText |
The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain greater than 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent Mr, probably as a result of glycosylation differences.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-1970935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-2156742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-2264840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-2271582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-2348864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-2540699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-2692709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-3004742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-3280584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-3553425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-3597413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-3839598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-6421318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-6890381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-7295669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1590751-903365
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
283 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
643-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1590751-Animals,
pubmed-meshheading:1590751-Baculoviridae,
pubmed-meshheading:1590751-Blotting, Western,
pubmed-meshheading:1590751-Cell Line,
pubmed-meshheading:1590751-Cell Membrane,
pubmed-meshheading:1590751-Cytochalasin B,
pubmed-meshheading:1590751-DNA, Recombinant,
pubmed-meshheading:1590751-Erythrocytes,
pubmed-meshheading:1590751-Fluorescent Antibody Technique,
pubmed-meshheading:1590751-Gene Expression,
pubmed-meshheading:1590751-Genetic Vectors,
pubmed-meshheading:1590751-Glycosylation,
pubmed-meshheading:1590751-Humans,
pubmed-meshheading:1590751-Molecular Weight,
pubmed-meshheading:1590751-Monosaccharide Transport Proteins,
pubmed-meshheading:1590751-Moths,
pubmed-meshheading:1590751-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
Characterization of functional human erythrocyte-type glucose transporter (GLUT1) expressed in insect cells using a recombinant baculovirus.
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pubmed:affiliation |
Department of Biochemistry, Royal Free Hospital School of Medicine, University of London, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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