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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-7-6
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pubmed:abstractText |
The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0302-766X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15906100-Animals,
pubmed-meshheading:15906100-Biological Markers,
pubmed-meshheading:15906100-Cholecystokinin,
pubmed-meshheading:15906100-Chromogranin A,
pubmed-meshheading:15906100-Chromogranins,
pubmed-meshheading:15906100-Gastric Mucosa,
pubmed-meshheading:15906100-Gastrin-Secreting Cells,
pubmed-meshheading:15906100-Gastrins,
pubmed-meshheading:15906100-Gene Expression Regulation,
pubmed-meshheading:15906100-Immunohistochemistry,
pubmed-meshheading:15906100-Mice,
pubmed-meshheading:15906100-Mice, Transgenic,
pubmed-meshheading:15906100-Microscopy, Electron,
pubmed-meshheading:15906100-Proprotein Convertase 1,
pubmed-meshheading:15906100-RNA, Messenger,
pubmed-meshheading:15906100-Radioimmunoassay,
pubmed-meshheading:15906100-Reverse Transcriptase Polymerase Chain Reaction
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pubmed:year |
2005
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pubmed:articleTitle |
Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals.
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pubmed:affiliation |
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark. lfh@rh.dk
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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