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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-7-6
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pubmed:abstractText |
An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10-500 ng ml(-1).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1618-2642
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
382
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1265-8
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pubmed:meshHeading |
pubmed-meshheading:15906007-Calibration,
pubmed-meshheading:15906007-Chromatography, High Pressure Liquid,
pubmed-meshheading:15906007-Cyclization,
pubmed-meshheading:15906007-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:15906007-Humans,
pubmed-meshheading:15906007-Lactones,
pubmed-meshheading:15906007-Magnetic Resonance Spectroscopy,
pubmed-meshheading:15906007-Molecular Structure,
pubmed-meshheading:15906007-Phenanthrenes,
pubmed-meshheading:15906007-Reference Standards,
pubmed-meshheading:15906007-Reproducibility of Results,
pubmed-meshheading:15906007-Sensitivity and Specificity,
pubmed-meshheading:15906007-Sulfones,
pubmed-meshheading:15906007-Ultraviolet Rays
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pubmed:year |
2005
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pubmed:articleTitle |
Pre-column derivatization of rofecoxib for determination in serum by HPLC.
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pubmed:affiliation |
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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