Linked Life Data

15905076

Source:http://linkedlifedata.com/resource/pubmed/id/15905076

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-6-7
pubmed:abstractText
E-cadherin is a member of the classical cadherin family and is known to be involved in cell-cell adhesion and the adhesion-dependent morphogenesis of various tissues. We isolated a zebrafish mutant (cdh1(rk3)) that has a mutation in the e-cadherin/cdh1 gene. The mutation rk3 is a hypomorphic allele, and the homozygous mutant embryos displayed variable phenotypes in gastrulation and tissue morphogenesis. The most severely affected embryos displayed epiboly delay, decreased convergence and extension movements, and the dissociation of cells from the embryos, resulting in early embryonic lethality. The less severely affected embryos survived through the pharyngula stage and showed flattened anterior neural tissue, abnormal positioning and morphology of the hatching gland, scattered trigeminal ganglia, and aberrant axon bundles from the trigeminal ganglia. Maternal-zygotic cdh1(rk3) embryos displayed epiboly arrest during gastrulation, in which the enveloping layer (EVL) and the yolk syncytial layer but not the deep cells (DC) completed epiboly. A similar phenotype was observed in embryos that received antisense morpholino oligonucleotides (cdh1MO) against E-cadherin, and in zebrafish epiboly mutants. Complementation analysis with the zebrafish epiboly mutant weg suggested that cdh1(rk3) is allelic to half baked/weg. Immunohistochemistry with an anti-beta-catenin antibody and electron microscopy revealed that adhesion between the DCs and the EVL was mostly disrupted but the adhesion between DCs was relatively unaffected in the MZcdh1(rk3) mutant and cdh1 morphant embryos. These data suggest that E-cadherin-mediated cell adhesion between the DC and EVL plays a role in the epiboly movement in zebrafish.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
747-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15905076-Alleles, pubmed-meshheading:15905076-Animals, pubmed-meshheading:15905076-Base Sequence, pubmed-meshheading:15905076-Boron Compounds, pubmed-meshheading:15905076-Cadherins, pubmed-meshheading:15905076-Cell Adhesion, pubmed-meshheading:15905076-Cell Movement, pubmed-meshheading:15905076-Exons, pubmed-meshheading:15905076-Gastrula, pubmed-meshheading:15905076-Gene Expression Regulation, Developmental, pubmed-meshheading:15905076-Immunohistochemistry, pubmed-meshheading:15905076-In Situ Hybridization, pubmed-meshheading:15905076-Indoles, pubmed-meshheading:15905076-Microscopy, Electron, pubmed-meshheading:15905076-Molecular Sequence Data, pubmed-meshheading:15905076-Movement, pubmed-meshheading:15905076-Mutation, pubmed-meshheading:15905076-Neurons, pubmed-meshheading:15905076-Oligonucleotides, pubmed-meshheading:15905076-Phenotype, pubmed-meshheading:15905076-Sequence Homology, Nucleic Acid, pubmed-meshheading:15905076-Temperature, pubmed-meshheading:15905076-Zebrafish, pubmed-meshheading:15905076-Zygote
pubmed:year
2005
pubmed:articleTitle
E-cadherin is required for gastrulation cell movements in zebrafish.
pubmed:affiliation
Laboratory for Vertebrate Axis Formation, Center for Developmental Biology, RIKEN, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't