Linked Life Data

15902706

Source:http://linkedlifedata.com/resource/pubmed/id/15902706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-5-25
pubmed:abstractText
In order to identify patients at risk for developing post-transplant lymphoproliferative disease (PTLD), a sensitive nested RT-PCR method for detection of EBNA1 gene expression in peripheral blood cells was used. EBNA1 expression in peripheral blood samples from 60 organ recipients was analyzed and compared with 24 healthy controls in a retrospective study. Overall, EBNA1-positive samples were detected at least once in 43% of the transplant patients with post-transplant lymphoproliferative disease, in 18% of the other transplant patients and in none of the healthy controls. The odds ratio for EBNA1 expression in patients with post-transplant lymphoproliferative disease was 3.42 (95% CI=1.02-11.54) compared to other transplant recipients. Together with normal EBV Q promoter initiated EBNA1 transcripts, an alternatively spliced form was expressed in peripheral blood cells in the above-mentioned transplant patients. This transcript lacks the U leader exon in the 5'-untranslated region (UTR). We have previously identified and characterized a functional internal ribosome entry site, the EBNA IRES, in the untranslated U leader exon of EBNA1. Transfection experiments with EBNA1 coding plasmids followed by Western blot showed that the EBNA IRES promotes cap-independent translation and increases the EBNA1 protein level. The alternative EBNA1 transcript lacking this function is expressed in the majority of the investigated EBNA1-positive patient samples as well as in some EBV-positive B-cell lines. Alternative splicing in this form gives EBV potential to regulate the translation of EBNA1 by modifying the 5' UTR. These findings indicate a new mechanism for EBNA1 expression in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0146-6615
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
378-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15902706-5' Untranslated Regions, pubmed-meshheading:15902706-Adolescent, pubmed-meshheading:15902706-Adult, pubmed-meshheading:15902706-Alternative Splicing, pubmed-meshheading:15902706-Blotting, Western, pubmed-meshheading:15902706-Cell Line, pubmed-meshheading:15902706-Child, pubmed-meshheading:15902706-Child, Preschool, pubmed-meshheading:15902706-Epstein-Barr Virus Nuclear Antigens, pubmed-meshheading:15902706-Exons, pubmed-meshheading:15902706-Female, pubmed-meshheading:15902706-Herpesvirus 4, Human, pubmed-meshheading:15902706-Humans, pubmed-meshheading:15902706-Infant, pubmed-meshheading:15902706-Leukocytes, pubmed-meshheading:15902706-Male, pubmed-meshheading:15902706-Middle Aged, pubmed-meshheading:15902706-Organ Transplantation, pubmed-meshheading:15902706-RNA, Messenger, pubmed-meshheading:15902706-RNA, Viral, pubmed-meshheading:15902706-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15902706-Sweden, pubmed-meshheading:15902706-Transfection
pubmed:year
2005
pubmed:articleTitle
Alternative EBNA1 expression in organ transplant patients.
pubmed:affiliation
Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg University, Gothenburg, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't