Linked Life Data

15901927

Source:http://linkedlifedata.com/resource/pubmed/id/15901927

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2005-5-19
pubmed:abstractText
For effective cancer chemotherapy, chemosensitivity testing of anticancer drugs should be performed using fresh surgical specimens obtained from the cancer. We have developed a new in vitro chemosensitivity test named the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The CD-DST method consists of a collagen gel droplet embedded culture step, exposure and washout of anticancer drug, a serum-free culture step, and evaluation of anticancer effect by image analysis. This method has many advantages including a high success rate for primary culture, the need for only a small number of cells for the test, easy quantification of the anticancer effects without contamination with fibroblasts by using an image analysis system, a good correlation between in vitro and in vivo results, and simplicity and speed. The CD-DST method can be performed in the laboratory using a system kit Primaster.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1543-1894
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-67
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Collagen gel droplet culture method to examine in vitro chemosensitivity.
pubmed:affiliation
Biochemical Laboratory, Nitta Gelatin Inc., Futamata, Yao-City, Japan.
pubmed:publicationType
Journal Article