Linked Life Data

15899964

Source:http://linkedlifedata.com/resource/pubmed/id/15899964

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-6-2
pubmed:abstractText
Transcriptional regulatory elements play essential roles in gene expression during animal development and cellular response to environmental signals, but our knowledge of these regions in the human genome is limited despite the availability of the complete genome sequence. Promoters mark the start of every transcript and are an important class of regulatory elements. A large, complex protein structure known as the pre-initiation complex (PIC) is assembled on all active promoters, and the presence of these proteins distinguishes promoters from other sequences in the genome. Using components of the PIC as tags, we isolated promoters directly from human cells as protein-DNA complexes and identified the resulting DNA sequences using genomic tiling microarrays. Our experiments in four human cell lines uncovered 252 PIC-binding sites in 44 semirandomly selected human genomic regions comprising 1% (30 megabase pairs) of the human genome. Nearly 72% of the identified fragments overlap or immediately flank 5' ends of known cDNA sequences, while the remainder is found in other genomic regions that likely harbor putative promoters of unannotated transcripts. Indeed, molecular analysis of the RNA isolated from one cell line uncovered transcripts initiated from over half of the putative promoter fragments, and transient transfection assays revealed promoter activity for a significant proportion of fragments when they were fused to a luciferase reporter gene. These results demonstrate the specificity of a genome-wide analysis method for mapping transcriptional regulatory elements and also indicate that a small, yet significant number of human genes remains to be discovered.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-10547847, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-10657304, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-11125145, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-11206552, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-11498575, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-11823631, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-11988577, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12110178, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12399584, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12519940, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12519942, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12519945, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12566409, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12651739, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-12808131, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14523437, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14527297, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14527995, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14580351, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14661024, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14681363, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14702039, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14707170, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14744438, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14980218, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14988562, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-14993201, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15082775, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15114340, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15123803, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15145350, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15175259, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15338621, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15343339, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15489334, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15496913, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-15499007, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-2472398, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-7680771, http://linkedlifedata.com/resource/pubmed/commentcorrection/15899964-8125298
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1088-9051
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
830-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Direct isolation and identification of promoters in the human genome.
pubmed:affiliation
Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural