Linked Life Data

15897802

Source:http://linkedlifedata.com/resource/pubmed/id/15897802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-5-17
pubmed:abstractText
Control of dendritic cell (DC) function is critical for strategies to modulate innate and acquired immune responses. We examined whether transduction of murine DCs with adenoviral vectors (Adv) expressing interleukin (IL)-10 could alter their phenotype and T cell stimulatory function. Murine bone marrow-derived DCs were transduced with AdV encoding human IL-10 or green fluorescent protein (GFP). Whereas transduction of immature DCs with AdV/GFP resulted in dose-dependent maturation, DCs transduced with Adv/IL-10 maintained an immature state with low major histocompatibility complex (MHC) class II, CD86, and IL-12 expression. The Adv/IL-10 transduced DCs were phenotypically unique, characterized by suppression of IL-12 expression, failure to stimulate Th1 or Th2 cytokine responses, and retained capacity to endocytose antigen. Importantly, Adv/IL-10-transduced DCs were biologically active in vivo, in that administration of these DCs into mice before a generalized peritonitis significantly improved survival. We conclude that Adv/IL-10 transduction of DCs provides an efficient means to modulate DC function. The capacity to modify DCs by adenoviral expression of IL-10 may provide a novel ex vivo or in vivo approach to mitigate acute and chronic inflammatory diseases like sepsis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
507-15
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15897802-Adenoviridae, pubmed-meshheading:15897802-Animals, pubmed-meshheading:15897802-Antigens, CD, pubmed-meshheading:15897802-Antigens, CD3, pubmed-meshheading:15897802-Antigens, CD40, pubmed-meshheading:15897802-Antigens, CD86, pubmed-meshheading:15897802-Cell Survival, pubmed-meshheading:15897802-Cells, Cultured, pubmed-meshheading:15897802-Coculture Techniques, pubmed-meshheading:15897802-Dendritic Cells, pubmed-meshheading:15897802-Endocytosis, pubmed-meshheading:15897802-Female, pubmed-meshheading:15897802-Flow Cytometry, pubmed-meshheading:15897802-Gene Therapy, pubmed-meshheading:15897802-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:15897802-Green Fluorescent Proteins, pubmed-meshheading:15897802-Interleukin-10, pubmed-meshheading:15897802-Interleukin-12, pubmed-meshheading:15897802-Lipopolysaccharides, pubmed-meshheading:15897802-Lymph Nodes, pubmed-meshheading:15897802-Lymphocytes, pubmed-meshheading:15897802-Membrane Glycoproteins, pubmed-meshheading:15897802-Mice, pubmed-meshheading:15897802-Mice, Inbred C57BL, pubmed-meshheading:15897802-Phenotype, pubmed-meshheading:15897802-Sepsis, pubmed-meshheading:15897802-T-Lymphocytes, pubmed-meshheading:15897802-Th1 Cells, pubmed-meshheading:15897802-Th2 Cells, pubmed-meshheading:15897802-Time Factors
pubmed:year
2005
pubmed:articleTitle
Functional modification of dendritic cells with recombinant adenovirus encoding interleukin 10 for the treatment of sepsis.
pubmed:affiliation
Department of Surgery, University of Florida College of Medicine, Gainesville, Florida 32610, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural