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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-10-24
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pubmed:abstractText |
The present study examined whether lysophosphatidic acid (LPA) induces histamine release and the role of Rho-associated protein kinase (ROCK) in histamine release utilizing Y-27632, an inhibitor of ROCK. Rat peritoneal mast cells and mouse skin fragments were challenged with LPA; subsequently, histamine contents were measured by spectrofluorometric assay. LPA-induced histamine release from mast cells and skin fragments occurred in a time- and dose-dependent manner Pretreatment with Y-27632 inhibited LPA-induced histamine release in a dose-dependent fashion. LPA-induced histamine release was not influenced by calcium-free conditions; however, A23187-induced histamine release decreased significantly. TMB-8, an intracellular calcium antagonist, dose-dependently inhibited the histamine release under these conditions. Additionally, Y-27632 scarcely affected A23187-induced histamine release. These findings suggest that LPA-induced histamine release may be attributable to calcium release from intracellular stores. Moreover, ROCK participates in the extracellular calcium-independent process of LPA-induced histamine release.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-(N,N-diethylamino)octyl-3,4,5-trim...,
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Gallic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Y 27632,
http://linkedlifedata.com/resource/pubmed/chemical/lysophosphatidic acid,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0031-7012
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pubmed:author |
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pubmed:copyrightInfo |
Copyright (c) 2005 S. Karger AG, Basel.
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-20
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15897679-Amides,
pubmed-meshheading:15897679-Animals,
pubmed-meshheading:15897679-Calcimycin,
pubmed-meshheading:15897679-Calcium,
pubmed-meshheading:15897679-Calcium Channel Blockers,
pubmed-meshheading:15897679-Dose-Response Relationship, Drug,
pubmed-meshheading:15897679-Enzyme Inhibitors,
pubmed-meshheading:15897679-Gallic Acid,
pubmed-meshheading:15897679-Histamine Release,
pubmed-meshheading:15897679-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:15897679-Ionophores,
pubmed-meshheading:15897679-Lysophospholipids,
pubmed-meshheading:15897679-Male,
pubmed-meshheading:15897679-Mast Cells,
pubmed-meshheading:15897679-Mice,
pubmed-meshheading:15897679-Mice, Inbred ICR,
pubmed-meshheading:15897679-Peritoneum,
pubmed-meshheading:15897679-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15897679-Pyridines,
pubmed-meshheading:15897679-Rats,
pubmed-meshheading:15897679-Rats, Sprague-Dawley,
pubmed-meshheading:15897679-Skin,
pubmed-meshheading:15897679-Species Specificity,
pubmed-meshheading:15897679-Time Factors,
pubmed-meshheading:15897679-rho-Associated Kinases
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pubmed:year |
2005
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pubmed:articleTitle |
Lysophosphatidic acid induces histamine release from mast cells and skin fragments.
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pubmed:affiliation |
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
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