Linked Life Data

15896659

Source:http://linkedlifedata.com/resource/pubmed/id/15896659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-5-17
pubmed:abstractText
Peroxisome proliferator-activated receptor-gamma (PPARgamma) plays a role in adipocyte differentiation and insulin sensitization. We identified and characterized a new C/T substitution at position -689 (-689C>T) in the P2 promoter of PPARgamma in a putative GATA binding site. By electrophoretic mobility shift assay, both GATA2 and GATA3 proteins could bind weakly to the wild-type P2 -689 GATA binding site but not to the mutated site. Neither GATA2 nor GATA3 was able to regulate significantly the P2 promoter activity in a reporter-luciferase assay, whatever the allele at position -689 was, suggesting that the -689 putative GATA site was probably not a functional target for GATAs. However, the presence of the -689T allele rendered the P2 promoter less active at the basal state. We genotyped a population of 1155 men and women for the -689C>T polymorphism and looked for possible associations with anthropometric and lipid variables. The carriers of the -689T allele had elevated body weight and LDL-cholesterol concentrations compared with the homozygous for the common allele. Haplotype analyses including the -681C>G (P3 promoter), -689C>T (P2 promoter), and Pro12Ala (exon B) polymorphisms were performed. Carriers of the G-T-Ala haplotype (corresponding to the P3 -681C>G, P2 -689C>T and Pro12Ala polymorphisms in this order) had elevated LDL-cholesterol concentrations and body weight compared with C-C-Pro individuals. In conclusion, we identified a new polymorphism in the P2 promoter of PPARgamma. The P3 -681C>G, P2 -689C>T, and Pro12Ala polymorphisms and related haplotypes were associated with higher body weight and plasma LDL-cholesterol concentrations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1096-7192
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
140-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15896659-Adult, pubmed-meshheading:15896659-Alleles, pubmed-meshheading:15896659-Animals, pubmed-meshheading:15896659-Body Weight, pubmed-meshheading:15896659-COS Cells, pubmed-meshheading:15896659-Cercopithecus aethiops, pubmed-meshheading:15896659-Cholesterol, LDL, pubmed-meshheading:15896659-DNA-Binding Proteins, pubmed-meshheading:15896659-Electrophoretic Mobility Shift Assay, pubmed-meshheading:15896659-Female, pubmed-meshheading:15896659-France, pubmed-meshheading:15896659-GATA2 Transcription Factor, pubmed-meshheading:15896659-GATA3 Transcription Factor, pubmed-meshheading:15896659-Haplotypes, pubmed-meshheading:15896659-Humans, pubmed-meshheading:15896659-Linkage Disequilibrium, pubmed-meshheading:15896659-Male, pubmed-meshheading:15896659-Middle Aged, pubmed-meshheading:15896659-PPAR gamma, pubmed-meshheading:15896659-Polymorphism, Genetic, pubmed-meshheading:15896659-Promoter Regions, Genetic, pubmed-meshheading:15896659-Trans-Activators, pubmed-meshheading:15896659-Transcription Factors
pubmed:year
2005
pubmed:articleTitle
Study of a new PPARgamma2 promoter polymorphism and haplotype analysis in a French population.
pubmed:affiliation
INSERM, U508, Institut Pasteur de Lille, 1 rue du Pr. Calmette, BP 245, Lille Cedex F-59019, France. Aline.Meirhaeghe-Hurez@pasteur-lille.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't