Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-6-13
pubmed:abstractText
The tooth pulp innervation originates from the trigeminal ganglion (TG) and represents an illustrative example of tissue targeting by sensory nerves. Pulpal fibroblasts strongly promote neurite outgrowth from TG neurons in vitro. In the present study, we have investigated the possible participation of laminins (LNs), potent neuritogenic extracellular matrix components. Immunohistochemistry of human tooth pulp demonstrated expression of LN alpha1, alpha2, alpha4, alpha5, beta1 and gamma1, and laminin-binding integrin alpha3, alpha6, beta1 and beta4 chains in nerves. Though faintly stained for laminins in situ, pulpal fibroblasts reacted, once cultured and permeabilized, with antibodies to LN alpha2, alpha4, beta1 and gamma1 chains by flow cytometry. The cells also expressed the corresponding mRNAs and were able to assemble and secrete LN-2 (alpha2beta1gamma1, Lm-211) and LN-8 (alpha4beta1gamma1, Lm-411). LN-8 displayed a chondroitin sulphate (CS) modification in its alpha4 chain. In functional assays, mouse LN-1 (alpha1beta1gamma1, Lm-111) and recombinant human (rh) LN-8, but not native or rhLN-2, strongly promoted neurite outgrowth from TG neurons, mimicking the effect of cultured pulp fibroblast. Altogether, the results indicate that LN-2 and LN-8 are synthesized by tooth pulp fibroblasts and differentially promote neurite outgrowth from TG neurons. LN-8 may contribute to sensory innervation of teeth and other tissues during development and/or regeneration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Chondroitin Sulfates, http://linkedlifedata.com/resource/pubmed/chemical/Integrins, http://linkedlifedata.com/resource/pubmed/chemical/LAMA4 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/LAMB1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Lama4 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Lamb1-1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Laminin, http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms, http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits, http://linkedlifedata.com/resource/pubmed/chemical/laminin 8, http://linkedlifedata.com/resource/pubmed/chemical/laminin alpha 2, http://linkedlifedata.com/resource/pubmed/chemical/laminin gamma 1
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
307
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
329-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15894315-Adolescent, pubmed-meshheading:15894315-Adult, pubmed-meshheading:15894315-Animals, pubmed-meshheading:15894315-Cell Enlargement, pubmed-meshheading:15894315-Cells, Cultured, pubmed-meshheading:15894315-Chondroitin Sulfates, pubmed-meshheading:15894315-Dental Pulp, pubmed-meshheading:15894315-Fibroblasts, pubmed-meshheading:15894315-Flow Cytometry, pubmed-meshheading:15894315-Gene Expression, pubmed-meshheading:15894315-Humans, pubmed-meshheading:15894315-Immunohistochemistry, pubmed-meshheading:15894315-Integrins, pubmed-meshheading:15894315-Laminin, pubmed-meshheading:15894315-Mice, pubmed-meshheading:15894315-Neurites, pubmed-meshheading:15894315-Neurons, pubmed-meshheading:15894315-Protein Isoforms, pubmed-meshheading:15894315-Protein Subunits, pubmed-meshheading:15894315-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15894315-Trigeminal Ganglion
pubmed:year
2005
pubmed:articleTitle
Laminins 2 (alpha2beta1gamma1, Lm-211) and 8 (alpha4beta1gamma1, Lm-411) are synthesized and secreted by tooth pulp fibroblasts and differentially promote neurite outgrowth from trigeminal ganglion sensory neurons.
pubmed:affiliation
Center for Oral Biology, Department of Odontology, Karolinska Institutet at Huddinge, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't