Linked Life Data

15894285

Source:http://linkedlifedata.com/resource/pubmed/id/15894285

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-5-31
pubmed:abstractText
Recent studies suggest that there are strong parallels between development and patterning of the vertebrate vascular system and the nervous system. While previous observations reported generation of vascular and neuronal progenitors from embryonic stem (ES) cells, the question of parallel development of vascular and neuronal cells in the same culture has not yet been investigated. Mouse D3 ES cells were cultured for 4 days in differentiation medium IMDM with 15% FBS in 100 mm non-adhesive Petri dishes to allow cells to aggregate and form embryoid bodies. At day 5, fibronectin or all-trans retinoic acid with fibronectin was added to the culture. On day 9, the embryoid bodies were seeded on poly-L-ornithine/fibronectin-coated plates. After plating, half of the plates were treated with laminin for 3 days and maintained for 1 week in Neurobasal media with B27. Here we show that ES cells differentiate into interconnected rhythmically contracting aggregates of functional cardiomyocytes and neurons. Double immunofluorescence with anti-phospholamban, anti-SERCA2 antibodies to detect cardiomyocytes and with anti-MAP2 antibodies to detect neurons revealed the cell aggregates consisting entirely of cardiomyocytes with neuronal cells located on the periphery or covering the aggregate's surface. The observed concurrent development of cardiomyocytes and neurons suggests bidirectional communication between both cell types. We propose that crosstalk between cardiovascular and neuronal progenitors is an important mechanism for the development of both systems.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
332
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
653-6
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15894285-Animals, pubmed-meshheading:15894285-Calcium-Transporting ATPases, pubmed-meshheading:15894285-Cardiovascular System, pubmed-meshheading:15894285-Cell Aggregation, pubmed-meshheading:15894285-Cell Communication, pubmed-meshheading:15894285-Cell Differentiation, pubmed-meshheading:15894285-Cells, Cultured, pubmed-meshheading:15894285-Fibronectins, pubmed-meshheading:15894285-Laminin, pubmed-meshheading:15894285-Mice, pubmed-meshheading:15894285-Microtubule-Associated Proteins, pubmed-meshheading:15894285-Myocytes, Cardiac, pubmed-meshheading:15894285-Nervous System, pubmed-meshheading:15894285-Neurons, pubmed-meshheading:15894285-Pluripotent Stem Cells, pubmed-meshheading:15894285-Sarcoplasmic Reticulum Calcium-Transporting ATPases, pubmed-meshheading:15894285-Spheroids, Cellular, pubmed-meshheading:15894285-Tretinoin
pubmed:year
2005
pubmed:articleTitle
Parallel development of cardiomyocytes and neurons in embryonic stem cell culture.
pubmed:affiliation
Department of Physiology, The Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA. murashova1@mail.ecu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural