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rdf:type |
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lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0040649,
umls-concept:C0086860,
umls-concept:C0108555,
umls-concept:C0205245,
umls-concept:C0444669,
umls-concept:C1328695,
umls-concept:C1415927,
umls-concept:C1546857,
umls-concept:C1709390,
umls-concept:C1880022
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pubmed:issue |
4
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pubmed:dateCreated |
2005-5-16
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pubmed:abstractText |
Downstream core promoter elements are an expanding class of regulatory sequences that add considerable diversity to the promoter architecture of RNA polymerase II-transcribed genes. We set out to determine the factors necessary for downstream promoter element (DPE)-dependent transcription and find that, against expectations, TFIID and the GTFs are not sufficient. Instead, the protein kinase CK2 and the coactivator PC4 establish DPE-specific transcription in an in vitro transcription system containing TFIID, Mediator, and the GTFs. Chromatin immunoprecipitation analyses using the DPE-dependent IRF-1 and TAF7 promoters demonstrated that CK2, and PC4 are present on these promoters in vivo. In contrast, neither PC4 nor CK2 were detected on the TAF1-dependent cyclin D promoter, which contains a DCE type of downstream element. Our findings also demonstrate that CK2 activity alters TFIID-dependent recognition of DCE sequences. These data establish that CK2 acts as a switch, converting the transcriptional machinery from functioning on one type of downstream element to another.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Casein Kinase II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IFRD2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IRF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAF7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Binding Protein Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIID
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
471-81
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15893730-Casein Kinase II,
pubmed-meshheading:15893730-Cell Nucleus,
pubmed-meshheading:15893730-DNA-Binding Proteins,
pubmed-meshheading:15893730-Gene Expression Regulation,
pubmed-meshheading:15893730-Humans,
pubmed-meshheading:15893730-Immediate-Early Proteins,
pubmed-meshheading:15893730-Interferon Regulatory Factor-1,
pubmed-meshheading:15893730-Membrane Proteins,
pubmed-meshheading:15893730-Phosphoproteins,
pubmed-meshheading:15893730-Promoter Regions, Genetic,
pubmed-meshheading:15893730-Repressor Proteins,
pubmed-meshheading:15893730-TATA-Binding Protein Associated Factors,
pubmed-meshheading:15893730-Trans-Activators,
pubmed-meshheading:15893730-Transcription, Genetic,
pubmed-meshheading:15893730-Transcription Factor TFIID
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pubmed:year |
2005
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pubmed:articleTitle |
Functional characterization of core promoter elements: DPE-specific transcription requires the protein kinase CK2 and the PC4 coactivator.
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pubmed:affiliation |
Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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