Source:http://linkedlifedata.com/resource/pubmed/id/15890264
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-5-13
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| pubmed:databankReference | |
| pubmed:abstractText |
The present study shows that hemocytic granular cells synthesize and secrete type IV collagen (ColIV) in the silkworm Bombyx mori (B. mori) and suggests that these cells play roles in the formation of basement membrane, the encapsulation of foreign bodies, and the metamorphic remodeling of the gut. The full- and partial-length cDNA of B. mori prolyl 4-hydroxylase alpha subunit (BmP4Halpha) and B. mori ColIV (BmColIV) were cloned, respectively. In situ hybridization and immunocytochemistry on larval tissues and cells identified hemocytic granular cells as the cells that express mRNAs and proteins of both BmP4Halpha and BmColIV. Immunohistochemistry and immunocytochemistry demonstrated that BmColIV was present in the basement membrane and in the secretory granules of granular cells, respectively. Granular cells in culture secreted BmColIV without accompanying the degranulation and discharged it from the granules when the cells were degranulated. Nylon threads were inserted into the hemocoel of larvae. Granular cells concentrated around the nylon threads and encapsulated them as a self-defense reaction. BmColIV was found to be a component of the capsules. Furthermore, the present study showed that actively BmColIV-expressing granular cells accumulated around the midgut epithelium and formed BmColIV-rich thick basal lamina-like structures there in larval to pupal metamorphosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type IV,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Procollagen-Proline Dioxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Glucans
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0945-053X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
24
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
136-54
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| pubmed:meshHeading |
pubmed-meshheading:15890264-Amino Acid Sequence,
pubmed-meshheading:15890264-Animals,
pubmed-meshheading:15890264-Basement Membrane,
pubmed-meshheading:15890264-Bombyx,
pubmed-meshheading:15890264-Cloning, Molecular,
pubmed-meshheading:15890264-Collagen Type IV,
pubmed-meshheading:15890264-DNA, Complementary,
pubmed-meshheading:15890264-DNA Primers,
pubmed-meshheading:15890264-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15890264-Hemocytes,
pubmed-meshheading:15890264-Immunoblotting,
pubmed-meshheading:15890264-Immunohistochemistry,
pubmed-meshheading:15890264-Insects,
pubmed-meshheading:15890264-Metamorphosis, Biological,
pubmed-meshheading:15890264-Molecular Sequence Data,
pubmed-meshheading:15890264-Procollagen-Proline Dioxygenase,
pubmed-meshheading:15890264-RNA, Messenger,
pubmed-meshheading:15890264-Recombinant Proteins,
pubmed-meshheading:15890264-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15890264-Sequence Homology, Amino Acid,
pubmed-meshheading:15890264-Time Factors,
pubmed-meshheading:15890264-beta-Glucans
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| pubmed:year |
2005
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| pubmed:articleTitle |
Synthesis of prolyl 4-hydroxylase alpha subunit and type IV collagen in hemocytic granular cells of silkworm, Bombyx mori: Involvement of type IV collagen in self-defense reaction and metamorphosis.
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| pubmed:affiliation |
Yoshizato Project, Cooperative Link of Unique Science and Technology for Economy Revitalization, Hiroshima Prefectural Institute of Industrial Science and Technology, 3-10-32, Kagamiyama, Higashihiroshima, Hiroshima, 739-0046, Japan.
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| pubmed:publicationType |
Journal Article
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