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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2005-6-8
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pubmed:abstractText |
Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences that enabled us to determine that multiple acetylation occurs on the same molecule. At the same time, cleavages resulting in a terminal unacetylated lysine suggested that not all of these sites are fully modified. Using MS and MS/MS, we were able to characterize both the unmodified and acetylated tryptic peptides covering more than 82% of the protein.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1615-9853
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2288-96
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15887180-Acetylation,
pubmed-meshheading:15887180-Amino Acid Sequence,
pubmed-meshheading:15887180-Binding Sites,
pubmed-meshheading:15887180-Cell Cycle Proteins,
pubmed-meshheading:15887180-Histone Acetyltransferases,
pubmed-meshheading:15887180-Lysine,
pubmed-meshheading:15887180-Molecular Sequence Data,
pubmed-meshheading:15887180-Peptides,
pubmed-meshheading:15887180-Recombinant Proteins,
pubmed-meshheading:15887180-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:15887180-Transcription Factors,
pubmed-meshheading:15887180-p300-CBP Transcription Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Structural analysis of a highly acetylated protein using a curved-field reflectron mass spectrometer.
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pubmed:affiliation |
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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