Source:http://linkedlifedata.com/resource/pubmed/id/15885873
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2005-8-1
|
| pubmed:abstractText |
YCP, a mitogenic polysaccharide with its molecular weight (MW) of 2.4 x 10(3) kDa, was isolated from the mycelium of the marine filamentous fungus Phoma herbarum YS4108 by a combination of ion-exchange chromatography on DEAE-32 and gel permeation over Sephacryl S-400. The detailed compositional, spectroscopic and methylation analyses of the polysaccharide demonstrated that its backbone possessed most likely a linear alpha-(1 --> 4) bonded glucopyranoside main chain co-bearing through side alpha-(1 --> 6)-linkage. The alpha-(1 --> 4) bondage of the glucopyranoside building blocks in YCP was confirmed by the observation that it could be hydrolyzed by the alpha-amylase produced by Bacillus licheniformis. A reliable concentration monitoring experimentation highlighted that the reducing sugars released continuously from YCP during its incubation with the enzyme, and the MW of the main resulting fragment weighed 0.8 x 10(4) Da with approximately 10% of YCP converted to maltose, maltotriose and glucose after a 120-min enzymatic degradation. Finally, YCP was found to be able to increase phagocytic activity of mice in vitro and in vivo, indicating that it may be looked up as a potent immunomodulator that could activate macrophages.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
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| pubmed:issn |
0300-9084
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
87
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
747-54
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15885873-Animals,
pubmed-meshheading:15885873-Chromatography, Ion Exchange,
pubmed-meshheading:15885873-Dose-Response Relationship, Drug,
pubmed-meshheading:15885873-Enzyme Stability,
pubmed-meshheading:15885873-Female,
pubmed-meshheading:15885873-Fungi,
pubmed-meshheading:15885873-Hydrolysis,
pubmed-meshheading:15885873-Macrophages, Peritoneal,
pubmed-meshheading:15885873-Magnetic Resonance Spectroscopy,
pubmed-meshheading:15885873-Male,
pubmed-meshheading:15885873-Methylation,
pubmed-meshheading:15885873-Mice,
pubmed-meshheading:15885873-Mitogens,
pubmed-meshheading:15885873-Monosaccharides,
pubmed-meshheading:15885873-Phagocytosis,
pubmed-meshheading:15885873-Polysaccharides,
pubmed-meshheading:15885873-Spleen,
pubmed-meshheading:15885873-Time Factors,
pubmed-meshheading:15885873-alpha-Amylases
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Purification, characterization and enzymatic degradation of YCP, a polysaccharide from marine filamentous fungus Phoma herbarum YS4108.
|
| pubmed:affiliation |
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|