Source:http://linkedlifedata.com/resource/pubmed/id/15884775
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-5-11
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| pubmed:abstractText |
The advantage of freeze-dried mouse spermatozoa is that samples can be stored in the refrigerator (+4 degrees C). Moreover, the storage of freeze-dried spermatozoa at ambient temperature would permit spermatozoa to be shipped easily and at low cost around the world. To examine the influence of the storage temperature on freeze-dried spermatozoa, we assessed the fertilizing ability of spermatozoa stored at different temperatures. Cauda epididymal spermatozoa were freeze-dried in buffer consisting of 50 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, 50 mM NaCl, and 10 mM Tris-HCl (pH 8.0). Samples of freeze-dried spermatozoa were stored at -70, -20, +4, or +24 degrees C for periods of 1 week and 1, 3, and 5 months. Sperm chromosomes were maintained well at -70, -20, and + 4 degrees C for 5 months, and oocytes fertilized with these spermatozoa developed to normal offspring. Moreover, the chromosomal integrity of spermatozoa stored at -20 or + 4 degrees C did not decrease even after 17 months. In contrast, the chromosomes of spermatozoa stored at +24 degrees C were maintained well for 1 month but became considerably degraded after 3 months. In addition, to investigate the cause of deterioration of sperm chromosomes during storage at +24 degrees C, spermatozoa were freeze-dried in buffer containing DNase I. The chromosomes of spermatozoa freeze-dried with 1 or 0.2 units/ml of DNase I, 100% or 72%, respectively, exhibited chromosomal abnormalities. Our findings suggest that freeze-dried spermatozoa can be stored long-term with stability at +4 degrees C, and the suppression of nucleases present in the buffer or spermatozoa during storage led to the achievement of long-term storage of freeze-dried spermatozoa.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1532-0820
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
55
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
140-4
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15884775-Animal Husbandry,
pubmed-meshheading:15884775-Animals,
pubmed-meshheading:15884775-Chromosome Aberrations,
pubmed-meshheading:15884775-Cold Temperature,
pubmed-meshheading:15884775-Cryopreservation,
pubmed-meshheading:15884775-Embryo Transfer,
pubmed-meshheading:15884775-Embryonic Development,
pubmed-meshheading:15884775-Female,
pubmed-meshheading:15884775-Fertilization,
pubmed-meshheading:15884775-Fetal Development,
pubmed-meshheading:15884775-Male,
pubmed-meshheading:15884775-Mice,
pubmed-meshheading:15884775-Pregnancy,
pubmed-meshheading:15884775-Pregnancy Outcome,
pubmed-meshheading:15884775-Semen Preservation,
pubmed-meshheading:15884775-Sperm Injections, Intracytoplasmic,
pubmed-meshheading:15884775-Sperm-Ovum Interactions,
pubmed-meshheading:15884775-Spermatozoa
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| pubmed:year |
2005
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| pubmed:articleTitle |
Relation between storage temperature and fertilizing ability of freeze-dried mouse spermatozoa.
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| pubmed:affiliation |
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan.
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| pubmed:publicationType |
Journal Article
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