Linked Life Data

15884019

Source:http://linkedlifedata.com/resource/pubmed/id/15884019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-6-6
pubmed:abstractText
Cellular prion protein (PrP(C)) expression can be regulated by heat-shock stress, and we designed the present study to determine whether hypoglycemia could affect PrP(C) expression. RT-PCR and Western blotting were used to measure the expression of PrP(C) and heat-shock protein (Hsp70) in mouse neuroblastoma (N18) cells cultured 3 hr to 3 days in media deprived of 97.5% (L) or 75% (M) of its glucose. Hypoglycemia caused a concomitant time-dependent and glucose dose-dependent increase in PrP(C) and Hsp70. In addition, hypoglycemia also increased phosphorylated c-Jun N-terminal kinase (JNK) protein levels in a time-dependent manner. The upregulation of PrP(C) and Hsp70 under hypoglycemic conditions was disrupted by the specific JNK inhibitor SP600125. It was also found from in vitro studies that hypoglycemic conditions induced higher levels of PrP(C) promoter activity in PrP(C) promoters containing a heat-shock element (HSE) than in PrP(C) promoters lacking HSE. We propose that hypoglycemia-increased PrP(C) expression might be due to JNK phosphorylation of a heat-shock transcriptional factor, which then interacts with HSE in the promoter of PrP(C).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
887-94
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15884019-Animals, pubmed-meshheading:15884019-Blotting, Western, pubmed-meshheading:15884019-Cell Line, Tumor, pubmed-meshheading:15884019-Enzyme Inhibitors, pubmed-meshheading:15884019-Gene Expression, pubmed-meshheading:15884019-HSP70 Heat-Shock Proteins, pubmed-meshheading:15884019-Hypoglycemia, pubmed-meshheading:15884019-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:15884019-MAP Kinase Kinase 4, pubmed-meshheading:15884019-Mice, pubmed-meshheading:15884019-Mitogen-Activated Protein Kinase Kinases, pubmed-meshheading:15884019-Neuroblastoma, pubmed-meshheading:15884019-Phosphorylation, pubmed-meshheading:15884019-PrPC Proteins, pubmed-meshheading:15884019-Promoter Regions, Genetic, pubmed-meshheading:15884019-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2005
pubmed:articleTitle
Hypoglycemia enhances the expression of prion protein and heat-shock protein 70 in a mouse neuroblastoma cell line.
pubmed:affiliation
Neuro-Medical Scientific Center, Tzu-Chi Buddhist General Hospital, Hualien, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't