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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-6-13
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pubmed:abstractText |
The Escherichia coli thymidine kinase/thymidylate kinase (tk/tmk) fusion gene encodes an enzyme that efficiently converts the prodrug 3'-azido-2',3'-dideoxythymidine (AZT) into its toxic triphosphate derivative, a substance which stops DNA chain elongation. Integration of this marker gene into vaccinia virus that normally is not inhibited by AZT allowed the establishment of a powerful selection procedure for recombinant viruses. In contrast to the conventional vaccinia thymidine kinase (tk) selection that is performed in tk-negative cell lines, AZT selection can be performed in normal (tk-positive) cell lines. The technique is especially useful for the generation of replication-deficient vaccinia viruses and may also be used for gene knock-out studies of essential vaccinia genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
337
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
235-41
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:15882885-Animals,
pubmed-meshheading:15882885-Anti-HIV Agents,
pubmed-meshheading:15882885-Artificial Gene Fusion,
pubmed-meshheading:15882885-Biotransformation,
pubmed-meshheading:15882885-Cell Line,
pubmed-meshheading:15882885-Cercopithecus aethiops,
pubmed-meshheading:15882885-Escherichia coli,
pubmed-meshheading:15882885-Nucleoside-Phosphate Kinase,
pubmed-meshheading:15882885-Prodrugs,
pubmed-meshheading:15882885-Thymidine Kinase,
pubmed-meshheading:15882885-Vaccinia virus,
pubmed-meshheading:15882885-Zidovudine
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pubmed:year |
2005
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pubmed:articleTitle |
Dominant negative selection of vaccinia virus using a thymidine kinase/thymidylate kinase fusion gene and the prodrug azidothymidine.
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pubmed:affiliation |
Baxter BioScience/Vaccines, Biomedical Research Center, Uferstrasse 15, A-2304 Orth/Donau, Austria.
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pubmed:publicationType |
Journal Article
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