Linked Life Data

15879117

Source:http://linkedlifedata.com/resource/pubmed/id/15879117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-5-9
pubmed:abstractText
IFN-gamma-activated transcriptional element (GATE)-binding factor 1 (GBF1) was identified as a transactivator that induces gene expression through GATE, a novel IFN-inducible element. Although it can induce gene expression, it is an extremely weak DNA-binding protein on its own. GATE also binds another transcription factor, C/EBP-beta. Therefore, we explored whether GBF1 physically interacts with C/EBP-beta to induce IFN-gamma-regulated transcription. In response to IFN-gamma, C/EBP-beta undergoes phosphorylation at a critical ERK1/2 phosphorylation motif. Mutational inactivation of this motif and/or interference with the ERK1/2 activation prevented the IFN-gamma-induced interactions between GBF1 and C/EBP-beta. A 37-aa long peptide derived from the GBF1 protein can associate with C/EBP-beta in an IFN-inducible manner. These results identify a converging point for two transactivators that exert their effects through a single response element. Together, our studies identify a novel regulatory mechanism that controls IFN-induced transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6203-11
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15879117-Amino Acid Motifs, pubmed-meshheading:15879117-Amino Acid Sequence, pubmed-meshheading:15879117-Animals, pubmed-meshheading:15879117-CCAAT-Enhancer-Binding Protein-beta, pubmed-meshheading:15879117-Cell Line, pubmed-meshheading:15879117-Gene Expression Regulation, pubmed-meshheading:15879117-Humans, pubmed-meshheading:15879117-Immunologic Factors, pubmed-meshheading:15879117-Interferon-gamma, pubmed-meshheading:15879117-Intramolecular Oxidoreductases, pubmed-meshheading:15879117-Mice, pubmed-meshheading:15879117-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:15879117-Molecular Sequence Data, pubmed-meshheading:15879117-Protein Interaction Mapping, pubmed-meshheading:15879117-Protein Structure, Tertiary, pubmed-meshheading:15879117-Trans-Activators, pubmed-meshheading:15879117-Transfection
pubmed:year
2005
pubmed:articleTitle
IFN-gamma-stimulated transcriptional activation by IFN-gamma-activated transcriptional element-binding factor 1 occurs via an inducible interaction with CAAAT/enhancer-binding protein-beta.
pubmed:affiliation
Greenebaum Cancer Center, Department of Microbiology and Immunology, Molecular and Cellular Biology Graduate Program, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural