Source:http://linkedlifedata.com/resource/pubmed/id/15878373
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
28
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pubmed:dateCreated |
2005-5-9
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pubmed:abstractText |
Mesenchymal stromal cells (MSCs) seem to be a good alternative to chondrocytes for cartilage regeneration. To obtain new information on the sequence of cellular and molecular events during in vitro chondrogenic differentiation we analysed MSCs on a widely used hyaluronic acid biomaterial (Hyaff-11). Cellular differentiation was induced using two different concentrations of TGFbeta1 (10 and 20 ng/ml) and the process was analysed at different time points (24 h, and 7, 14, 21 and 28 days) using techniques of light and electron microscopy, real-time PCR and immunohistochemistry. We found that without TGFbeta MSCs did not survive while in the presence of TGFbeta the cells significantly proliferated from day 7 until day 28. Light and electron microscopy showed that TGFbeta at 20 ng/ml better induced the formation of cartilage-like tissue. Real-time PCR showed an increased expression of collagen type II, IX and aggrecan associated to a down-regulation of collagen type I. Immunohistochemical analysis confirmed that collagen type I was down-modulated while collagen type II increased from day 14 to day 28. These data clearly showed that higher concentrations of TGFbeta (20 ng/ml) induce chondrogenesis of MSCs on Hyaff-11 scaffold better than 10 ng/ml of TGFbeta. This process is characterized by a sequence of cellular and molecular events that deal with the in vitro formation of a cartilage-like tissue.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0142-9612
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pubmed:author |
pubmed-author:CavalloCarolaC,
pubmed-author:CristinoSandraS,
pubmed-author:FacchiniAndreaA,
pubmed-author:GrassiFrancescoF,
pubmed-author:LisignoliGinaG,
pubmed-author:Mario MaraldiNadirN,
pubmed-author:PiacentiniAnnaA,
pubmed-author:SolimandoLilianaL,
pubmed-author:ToneguzziStefaniaS,
pubmed-author:ZiniNicolettaN
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5677-86
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15878373-Biocompatible Materials,
pubmed-meshheading:15878373-Cell Differentiation,
pubmed-meshheading:15878373-Cell Proliferation,
pubmed-meshheading:15878373-Cell Survival,
pubmed-meshheading:15878373-Cells, Cultured,
pubmed-meshheading:15878373-Chondrocytes,
pubmed-meshheading:15878373-Chondrogenesis,
pubmed-meshheading:15878373-Humans,
pubmed-meshheading:15878373-Hyaluronic Acid,
pubmed-meshheading:15878373-Materials Testing,
pubmed-meshheading:15878373-Mesenchymal Stem Cells,
pubmed-meshheading:15878373-Stromal Cells,
pubmed-meshheading:15878373-Tissue Engineering
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pubmed:year |
2005
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pubmed:articleTitle |
Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold.
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pubmed:affiliation |
Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy. labimge@alma.unibo.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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