Linked Life Data

15877870

Source:http://linkedlifedata.com/resource/pubmed/id/15877870

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-5-9
pubmed:abstractText
Anti-angiogenic strategies are emerging as an important tool for the treatment of cancer and inflammatory diseases. In the present investigation we isolated several isoflavones from a tempeh (fermented soyabean) extract. The isolated isoflavones were identified as 5,7,4'-trihydroxyisoflavone (genistein), 7,4'-dihydroxyisoflavone (daidzein), 6,7,4'-trihydroxyisoflavone (factor 2), 7,8,4'-trihydroxyisoflavone (7,8,4'-TriOH) and 5,7,3',4'-tetrahydroxyisoflavone (orobol). The effects on angiogenesis of these isoflavones were evaluated in the chicken chorioallantoic membrane assay; their capacity to inhibit vascular endothelial growth factor-induced endothelial cell proliferation and expression of the Ets 1 transcription factor, known to be implicated in the regulation of new blood vessel formation, were also investigated. We found that all isoflavones inhibited angiogenesis, albeit with different potencies. Compared with negative controls, which slightly inhibited in vivo angiogenesis by 6.30 %, genistein reduced angiogenesis by 75.09 %, followed by orobol (67.96 %), factor 2 (56.77 %), daidzein (48.98 %) and 7,8,4'-TriOH (24.42 %). These compounds also inhibited endothelial cell proliferation, with orobol causing the greatest inhibition at lower concentrations. The isoflavones also inhibited Ets 1 expression, providing some insight into the molecular mechanisms of their action. Furthermore, the chemical structure of the different isoflavones suggests a structure-activity relationship. Our present findings suggest that the new isoflavones might be added to the list of low molecular mass therapeutic agents for the inhibition of angiogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0007-1145
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
317-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15877870-Angiogenesis Inhibitors, pubmed-meshheading:15877870-Animals, pubmed-meshheading:15877870-Cell Proliferation, pubmed-meshheading:15877870-Chick Embryo, pubmed-meshheading:15877870-Chorioallantoic Membrane, pubmed-meshheading:15877870-Endothelium, Vascular, pubmed-meshheading:15877870-Genistein, pubmed-meshheading:15877870-Humans, pubmed-meshheading:15877870-Isoflavones, pubmed-meshheading:15877870-Neovascularization, Pathologic, pubmed-meshheading:15877870-Proto-Oncogene Protein c-ets-1, pubmed-meshheading:15877870-Proto-Oncogene Proteins, pubmed-meshheading:15877870-Proto-Oncogene Proteins c-ets, pubmed-meshheading:15877870-Soy Foods, pubmed-meshheading:15877870-Structure-Activity Relationship, pubmed-meshheading:15877870-Transcription Factors, pubmed-meshheading:15877870-Vascular Endothelial Growth Factor A
pubmed:year
2005
pubmed:articleTitle
Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay.
pubmed:affiliation
Institute of Pathology, University of Bonn, Bonn, Germany. s.kiriakidis@imperial.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't