Source:http://linkedlifedata.com/resource/pubmed/id/15872055
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2005-5-5
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pubmed:abstractText |
Breast cancer is a major cause of cancer-related mortality in women. There are major discrepancies concerning the usefulness of various antibodies in detecting breast cancer susceptibility gene 1 (BRCA1) protein and its subcellular localization. The aim of the present study was to determine the specificity and sensitivity of immunohistochemistry (IHC) as a screening method for demonstrating BRCA1 expression. BRCA1 gene expression in archival paraffin-embedded breast cancer tissues was studied simultaneously at the protein and mRNA levels, and the two findings were compared. Forty-eight archival paraffin-embedded breast cancer tissues were studied for BRCA1 gene expression at protein level by IHC using four different antibodies against different BRCA1 epitopes and at mRNA level using real-time RT-PCR. BRCA1 mRNA expression was reduced or absent in 79% of the samples, and this finding correlated significantly with loss of BRCA1 protein expression in 83% of breast cancer tissues using one BRCA1 antibody studied (AB-1, against N-terminus epitope). The specificity of this antibody was 91.3%, and its sensitivity was 66.6%. There was no significant correlation between BRCA1 mRNA and protein expression as demonstrated by the remaining three antibodies. Antibody 8F7 had the highest sensitivity of 100%, but its specificity was 30.4% if mRNA levels were considered as the reference standard.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1554
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
621-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15872055-Adult,
pubmed-meshheading:15872055-Aged,
pubmed-meshheading:15872055-BRCA1 Protein,
pubmed-meshheading:15872055-Breast Neoplasms,
pubmed-meshheading:15872055-Female,
pubmed-meshheading:15872055-Humans,
pubmed-meshheading:15872055-Immunohistochemistry,
pubmed-meshheading:15872055-Middle Aged,
pubmed-meshheading:15872055-Paraffin Embedding,
pubmed-meshheading:15872055-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15872055-Sensitivity and Specificity
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pubmed:year |
2005
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pubmed:articleTitle |
BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry.
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pubmed:affiliation |
Department of Pathology, Molecular Pathology Laboratory, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, Kuwait. fahd@al-mulla.org
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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