Linked Life Data

15871462

Source:http://linkedlifedata.com/resource/pubmed/id/15871462

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-5-5
pubmed:databankReference
pubmed:abstractText
Mammalian imprinted genes, which are expressed from only one of the parental alleles, have a tendency to form clusters and are regulated by long-range mechanisms. Nuclear matrix-attachment regions (MARs), the anchor points of loop domains, are involved in coordination of gene expression and could play a role in regulation of imprinted domains. We have identified and mapped a total of 52 MARs in a 1-Mb imprinted domain on mouse distal chromosome 7 using our cosmid contigs and an in vitro MAR assay. We find two MAR clusters (comprising 20 and 19 MARs), one of which is mapped in the Th-Ins2 intergenic region, coincident with the boundary between the two imprinted subdomains. However, the imprinted/non-imprinted boundaries are not associated with a MAR. Based on the sequence information, we find that many of the MARs are rich in long interspersed nuclear elements. In addition, comparisons of the results obtained with several MAR-prediction software programs reveal good performance of ChrClass in terms of both sensitivity and specificity. This study presents the first large-scale mapping of MARs in an imprinted domain and provides a platform for understanding the roles of MARs in imprinting.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1340-2838
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
391-407
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15871462-Animals, pubmed-meshheading:15871462-Binding, Competitive, pubmed-meshheading:15871462-Cell Nucleus, pubmed-meshheading:15871462-Chromosome Mapping, pubmed-meshheading:15871462-Computer Simulation, pubmed-meshheading:15871462-Contig Mapping, pubmed-meshheading:15871462-Cosmids, pubmed-meshheading:15871462-Dose-Response Relationship, Drug, pubmed-meshheading:15871462-Gene Expression Regulation, pubmed-meshheading:15871462-Genomic Imprinting, pubmed-meshheading:15871462-Long Interspersed Nucleotide Elements, pubmed-meshheading:15871462-Matrix Attachment Regions, pubmed-meshheading:15871462-Mice, pubmed-meshheading:15871462-Models, Genetic, pubmed-meshheading:15871462-Molecular Sequence Data, pubmed-meshheading:15871462-Multigene Family, pubmed-meshheading:15871462-Nuclear Matrix, pubmed-meshheading:15871462-Protein Structure, Tertiary, pubmed-meshheading:15871462-Software
pubmed:year
2004
pubmed:articleTitle
Large-scale identification and mapping of nuclear matrix-attachment regions in the distal imprinted domain of mouse chromosome 7.
pubmed:affiliation
Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411-8540, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't