rdf:type |
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0018270,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0023418,
umls-concept:C0319852,
umls-concept:C0599956,
umls-concept:C1326205,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C2752151,
umls-concept:C2828366
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pubmed:issue |
6
|
pubmed:dateCreated |
2005-5-4
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pubmed:abstractText |
Cordyceps militaris is a traditional herbal ingredient, which has been used for patients suffering from cancer in Oriental medicine. In the present study, we investigated the biochemical mechanisms of anti-proliferative effects by aqueous extract of C. militaris (AECM) in human leukemia U937 cells. It was found that AECM could inhibit cell growth of U937 cells in a dose-dependent manner, which was associated with morphological change and apoptotic cell death such as formation of apoptotic bodies and DNA fragmentation. We observed the down-regulation of anti-apoptotic Bcl-2 expression and proteolytic activation of caspase-3 in AECM-treated U937 cells. However, AECM did not affect the pro-apoptotic Bax expression and activity of caspase-9. Furthermore, Western blotting and RT-PCR revealed that AECM treatment caused a dose-dependent inhibition of cyclooxygenase-2 and prostaglandin E2 accumulation. Taken together, these results indicated that the anti-proliferative effects of AECM were associated with the induction of apoptotic cell death through regulation of several major growth regulatory gene products such as Bcl-2 family expression and caspase protease activity, and AECM may have therapeutic potential in human leukemia treatment.
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
|
pubmed:issn |
1021-335X
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
13
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1211-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15870944-Apoptosis,
pubmed-meshheading:15870944-Blotting, Western,
pubmed-meshheading:15870944-Caspases,
pubmed-meshheading:15870944-Cell Proliferation,
pubmed-meshheading:15870944-Cordyceps,
pubmed-meshheading:15870944-Cyclooxygenase 2,
pubmed-meshheading:15870944-Dinoprostone,
pubmed-meshheading:15870944-Enzyme Activation,
pubmed-meshheading:15870944-Humans,
pubmed-meshheading:15870944-Membrane Proteins,
pubmed-meshheading:15870944-Plant Extracts,
pubmed-meshheading:15870944-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:15870944-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15870944-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15870944-U937 Cells,
pubmed-meshheading:15870944-bcl-2-Associated X Protein
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pubmed:year |
2005
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pubmed:articleTitle |
Growth inhibition of U937 leukemia cells by aqueous extract of Cordyceps militaris through induction of apoptosis.
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pubmed:affiliation |
Department of Oriental Medicine, Dongeui University College of Oriental Medicine, Biomedical Research Center of Oriental Medicine, Busan 614-052, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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