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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2005-5-3
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pubmed:abstractText |
Integrin-linked kinase (ILK) facilitates signal transduction between extracellular events and important intracellular survival pathways involving protein kinase B/Akt. We examined the role of ILK in determining pancreatic adenocarcinoma cellular chemoresistance to the nucleoside analogue gemcitabine. Cellular ILK expression was quantified by Western blot analysis. We examined the effects of overexpression of active ILK and of ILK knockdown induced by RNA interference on gemcitabine chemoresistance. We also examined the effects of modulating ILK expression on gemcitabine-induced caspase 3-mediated apoptosis, phosphorylation status of Akt (Ser473) and glycogen synthase kinase. Overexpression of ILK increased cellular gemcitabine chemoresistance, whereas ILK knockdown induced chemosensitization via increased caspase 3-mediated apoptosis. ILK knockdown attenuated Akt Ser473 and glycogen synthase kinase phosphorylation, whereas overexpression of constitutively active myristoylated Akt was sufficient to induce significant recovery in gemcitabine chemoresistance in the presence of ILK knockdown. Levels of ILK expression affect gemcitabine chemoresistance in pancreatic adenocarcinoma cells. This novel finding suggests that therapies directed against ILK and its downstream signaling targets may have the potential to enhance the efficacy of gemcitabine-based chemotherapy.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10029595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10389948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10523630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10637513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10712922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10949937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-10980607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11034087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11062251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11114721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11244511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11426535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11480565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11593435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-11595724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12481421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12670870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12671688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12686550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12771992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12778079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-12865934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-14532995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-14623342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-14661056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-14707728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-14749128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-14751515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-2111548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-8538749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-9196156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-9370076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-9539744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-9736715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15867245-9812896
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AKT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxycytidine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycogen Synthase Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/gemcitabine,
http://linkedlifedata.com/resource/pubmed/chemical/integrin-linked kinase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1078-0432
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3433-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15867245-Humans,
pubmed-meshheading:15867245-Serine,
pubmed-meshheading:15867245-Pancreatic Neoplasms,
pubmed-meshheading:15867245-Phosphorylation,
pubmed-meshheading:15867245-Adenocarcinoma,
pubmed-meshheading:15867245-Antimetabolites, Antineoplastic,
pubmed-meshheading:15867245-Cell Line, Tumor,
pubmed-meshheading:15867245-Cell Proliferation,
pubmed-meshheading:15867245-Inhibitory Concentration 50,
pubmed-meshheading:15867245-Deoxycytidine,
pubmed-meshheading:15867245-Drug Resistance, Neoplasm,
pubmed-meshheading:15867245-Transfection,
pubmed-meshheading:15867245-Apoptosis,
pubmed-meshheading:15867245-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15867245-Glycogen Synthase Kinases,
pubmed-meshheading:15867245-Blotting, Western,
pubmed-meshheading:15867245-Proto-Oncogene Proteins,
pubmed-meshheading:15867245-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:15867245-Caspases,
pubmed-meshheading:15867245-RNA, Small Interfering
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