Linked Life Data

1586660

Source:http://linkedlifedata.com/resource/pubmed/id/1586660

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-6-25
pubmed:abstractText
The effect of putative cholesterol 'precursors' on model membranes has been studied by deuterium nuclear, magnetic resonance (2H-NMR) spectroscopy. Oriented bilayers were prepared from 1-myristoyl-2-[2H27 myristoyl-sn-glycero-3-phosphocholine (DMPC-d27) and tricyclohexaprenols or octaprenediols. Order parameter profiles were determined and showed that tricyclohexaprenols and octaprenediols increase the acyl chain order in DMPC bilayers, but to a smaller extent than cholesterol. The order parameter increases, depending on the chain position, from 5% to 7% in the presence of ditertiary octaprenediol, and from 16% to 21% in the presence of tricyclohexaprenol-Z,Z. Aqueous multilamellar dispersions of DMPC-d27 and of DMPC-d27 containing 30 mol% tricyclohexaprenol-E,E were prepared, and the first moments calculated from 2H-NMR spectra over the temperature range 5-55 degrees C. Tricyclohexaprenol-E,E almost abolishes the phase transition of DMPC. Thus, as predicted, tricyclohexaprenols and octaprenediols have a cholesterol-like behaviour in lipid membranes; however their effect on the model DMPC system is weak. On the contrary, isoarborinol has no effect on the lipid chain order in the liquid-crystalline phase of DMPC bilayers. 2H-NMR spectra of aqueous dispersions of DMPC-d27 and 30 mol% isoarborinol between 25 and 60 degrees C showed the coexistence of two lamellar phases over a wide temperature range, which was confirmed by differential scanning calorimetry (DSC) and 31P-NMR spectroscopy. This absence of ordering effect of isoarborinol might be related to some inherent structural features.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
1105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-20
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
The interaction of various cholesterol 'ancestors' with lipid membranes: a 2H-NMR study on oriented bilayers.
pubmed:affiliation
Laboratoire de Chimie Organique des Substances Naturelles, associé au CNRS, Université Louis Pasteur, Strasbourg, France.
pubmed:publicationType
Journal Article