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rdf:type |
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|
lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
2005-7-4
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|
pubmed:abstractText |
The insertion of a DNA copy of its RNA genome into a chromosome of the host cell is mediated by the viral integrase with the help of mostly uncharacterized cellular cofactors. We have recently described that the transcriptional co-activator LEDGF/p75 strongly interacts with HIV-1 integrase. Here we show that interaction of HIV-1 integrase with LEDGF/p75 is important for viral replication. Using multiple approaches including two-hybrid interaction studies, random and directed mutagenesis, we could demonstrate that HIV-1 virus harboring a single mutation that disrupts integrase-LEDGF/p75 interaction, resulted in defective HIV-1 replication. Furthermore, we found that LEDGF/p75 tethers HIV-1 integrase to chromosomes and that this interaction may be important for the integration process and the replication of HIV-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:Ben-SlamaLiliaL,
pubmed-author:BenarousRichardR,
pubmed-author:BusschotsKatrienK,
pubmed-author:ChristFraukeF,
pubmed-author:DargemontCatherineC,
pubmed-author:DebyserZegerZ,
pubmed-author:EmilianiStéphaneS,
pubmed-author:MarounMarlèneM,
pubmed-author:MoisantFannyF,
pubmed-author:MousnierAurélieA,
pubmed-author:RainJean-ChristopheJC,
pubmed-author:TempéDenisD,
pubmed-author:Van MaeleBénédicteB,
pubmed-author:VandekerckhoveLinosL,
pubmed-author:WitvrouwMyriamM
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pubmed:issnType |
Print
|
|
pubmed:day |
8
|
|
pubmed:volume |
280
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
25517-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15855167-Chromosomes, Human,
pubmed-meshheading:15855167-Glutamine,
pubmed-meshheading:15855167-HIV Infections,
pubmed-meshheading:15855167-HIV Integrase,
pubmed-meshheading:15855167-HIV-1,
pubmed-meshheading:15855167-HeLa Cells,
pubmed-meshheading:15855167-Humans,
pubmed-meshheading:15855167-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:15855167-Virus Integration,
pubmed-meshheading:15855167-Virus Replication
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pubmed:year |
2005
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pubmed:articleTitle |
Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication.
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pubmed:affiliation |
Institut Cochin, U567 INSERM, UPR8104 CNRS, Université Paris VI et VII, Tour 43, 2e étage, couloir 43-44, 2 place Jussieu, F-75251 Paris cedex 05, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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