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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-6-16
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pubmed:abstractText |
The interleukin 1 (IL-1)-inducible mRNAs for plasminogen activator inhibitor type 2, manganese superoxide dismutase, and urokinase are overexpressed in old (greater than 70% of life-span completed) but not in young (less than 40% of life-span completed) human foreskin fibroblasts. Furthermore, the activity of this superoxide dismutase is greater in old than in young fibroblasts. IL-1 beta mRNA is detected by Northern blot analysis in old fibroblasts and its expression is further enhanced by a treatment with IL-1 alpha. IL-1 alpha and IL-1 beta mRNAs are detected in old foreskin and lung fibroblasts by a sensitive reverse transcription-PCR assay. IL-1 mRNA is consistently expressed after fibroblasts have completed 85% of their in vitro life-span; an assay with specific antibodies shows that IL-1 alpha is present in these fibroblasts. Prolonged treatment with IL-1 receptor antagonist decreases the levels of IL-1 alpha and of IL-1 alpha and IL-1 beta mRNAs. This observation suggests that IL-1 receptor antagonist inhibits an autocrine loop responsible for IL-1 expression. IL-1 mRNA accumulates in young fibroblasts treated with cycloheximide, suggesting that it is transcribed but unstable in these cells; accumulation of IL-1 mRNA in old fibroblasts may be due at least in part to increased stability. IL-1 alpha stimulates DNA synthesis in young fibroblasts but has progressively less effect as the cells age in culture. These data indicate that IL-1 is "constitutively" produced by aging fibroblasts and that IL-1 induces the expression of specific proteins in these cells. The mechanism for this constitutive production of IL-1 is explored in this paper.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-13905659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-14315085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1653242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1673967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1697682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1699607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1702541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1712899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1827201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1851231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1939146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-1993689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2104680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2137200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2137488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2139669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2166342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2204114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2218499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2391377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2419912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2421407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2500449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2522658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2537326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2574499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-2969618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3263703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3277884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3343230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3458185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3491091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3494807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-3495577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-4943714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-6325057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-6460819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-7350644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-7449839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-894030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584804-908739
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4683-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1584804-Humans,
pubmed-meshheading:1584804-Lung,
pubmed-meshheading:1584804-Skin,
pubmed-meshheading:1584804-Fibroblasts,
pubmed-meshheading:1584804-Base Sequence,
pubmed-meshheading:1584804-Cells, Cultured,
pubmed-meshheading:1584804-RNA, Messenger,
pubmed-meshheading:1584804-Urokinase-Type Plasminogen Activator,
pubmed-meshheading:1584804-Plasminogen Inactivators,
pubmed-meshheading:1584804-Molecular Sequence Data,
pubmed-meshheading:1584804-Transcription, Genetic,
pubmed-meshheading:1584804-Oligodeoxyribonucleotides,
pubmed-meshheading:1584804-Superoxide Dismutase,
pubmed-meshheading:1584804-Recombinant Proteins,
pubmed-meshheading:1584804-Interleukin-1,
pubmed-meshheading:1584804-Tumor Necrosis Factor-alpha,
pubmed-meshheading:1584804-Blotting, Northern,
pubmed-meshheading:1584804-Polymerase Chain Reaction
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