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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-6-16
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pubmed:databankReference |
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pubmed:abstractText |
Freshly ejaculated human semen has the appearance of a loose gel in which the predominant structural protein components are the seminal vesicle-secreted semenogelins (Sg). The primary structure of the 439-residue SgI has previously been obtained by cDNA cloning. This cDNA cross-hybridizes to a larger transcript coding for a second secretory protein, SgII. Here we report the almost complete structure of a precursor of SgII established by lambda gt11 clones isolated from epididymal and seminal vesicular cDNA libraries. The deduced amino acid sequence of the 559-residue mature protein has a molecular weight of 62,931 but an increase in weight may be provided by asparagine-linked oligosaccharide attachment at residue 249. SgII, which has 78% overall identity with SgI, contains eight 60-residue regions that display conspicuous internal sequence similarity, whereas SgI only contains six of these regions. The SgII structure is translated from an open reading frame in a polyadenylylated 2.4-kilobase transcript. The message is abundant in the seminal vesicles but rare in the epididymis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-1702714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2132066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2422647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2431723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2436946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2460148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2484712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2691882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2757795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2866578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-2912989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3477802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3611349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3676397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3691515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3691800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3706782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3902893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-3972122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-6164983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-6385215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-6484484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-6777776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-7099970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-7133997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584792-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4559-63
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1584792-Amino Acid Sequence,
pubmed-meshheading:1584792-Base Sequence,
pubmed-meshheading:1584792-Blotting, Northern,
pubmed-meshheading:1584792-Cloning, Molecular,
pubmed-meshheading:1584792-DNA,
pubmed-meshheading:1584792-Epididymis,
pubmed-meshheading:1584792-Gene Library,
pubmed-meshheading:1584792-Gonadal Steroid Hormones,
pubmed-meshheading:1584792-Humans,
pubmed-meshheading:1584792-Male,
pubmed-meshheading:1584792-Molecular Sequence Data,
pubmed-meshheading:1584792-Molecular Weight,
pubmed-meshheading:1584792-Oligodeoxyribonucleotides,
pubmed-meshheading:1584792-Protein Precursors,
pubmed-meshheading:1584792-Restriction Mapping,
pubmed-meshheading:1584792-Semen,
pubmed-meshheading:1584792-Seminal Plasma Proteins,
pubmed-meshheading:1584792-Seminal Vesicle Secretory Proteins,
pubmed-meshheading:1584792-Seminal Vesicles,
pubmed-meshheading:1584792-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1584792-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein.
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pubmed:affiliation |
Department of Clinical Chemistry, Lund University, Malmö General Hospital, Sweden.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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