Source:http://linkedlifedata.com/resource/pubmed/id/15847919
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2005-4-25
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| pubmed:abstractText |
Three multiplex hemi-nested RT-PCR assays were developed to detect simultaneously 12 RNA respiratory viruses: influenza viruses A, B and C, human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV), parainfluenza virus types 1-4 (PIV-1, -2, -3 and -4), human coronavirus OC43 and 229E (HCoV) and rhinovirus (hRV). An internal amplification control was included in one of the RT-PCR assays. The RT-PCR multiplex 1 and the hemi-nested multiplex 1 detected 1 and 0.1 TCID50 of RSV A, respectively, and 0.01 and 0.001 TCID50 of influenza virus A/H3N2, respectively. Two hundred and three nasal aspirates from hospitalised children were retrospectively tested in comparison with two conventional methods: direct immunofluorescence assay and viral isolation technique. Almost all samples (89/91) that were positive by immunofluorescence assay and/or viral isolation technique were detected by the multiplex assay. This method also detected an additional 85 viruses and 33 co-infections. The overall sensitivity (98%), rapidity and enhanced efficiency of these multiplex hemi-nested RT-PCR assays suggest that they would be a significant improvement over conventional methods for the detection of a broad spectrum of respiratory viruses.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0166-0934
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
126
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
53-63
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15847919-Child,
pubmed-meshheading:15847919-Coronavirus 229E, Human,
pubmed-meshheading:15847919-Coronavirus OC43, Human,
pubmed-meshheading:15847919-Fluorescent Antibody Technique,
pubmed-meshheading:15847919-Humans,
pubmed-meshheading:15847919-Influenza A virus,
pubmed-meshheading:15847919-Influenza B virus,
pubmed-meshheading:15847919-Influenzavirus C,
pubmed-meshheading:15847919-Metapneumovirus,
pubmed-meshheading:15847919-Nasal Cavity,
pubmed-meshheading:15847919-Parainfluenza Virus 1, Human,
pubmed-meshheading:15847919-Parainfluenza Virus 2, Human,
pubmed-meshheading:15847919-Parainfluenza Virus 3, Human,
pubmed-meshheading:15847919-Parainfluenza Virus 4, Human,
pubmed-meshheading:15847919-Quality Control,
pubmed-meshheading:15847919-RNA, Viral,
pubmed-meshheading:15847919-RNA Viruses,
pubmed-meshheading:15847919-Respiratory Syncytial Viruses,
pubmed-meshheading:15847919-Respiratory Tract Infections,
pubmed-meshheading:15847919-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15847919-Rhinovirus,
pubmed-meshheading:15847919-Sensitivity and Specificity,
pubmed-meshheading:15847919-Virus Cultivation
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses.
|
| pubmed:affiliation |
Laboratory of Human and Molecular Virology, University Hospital, av. Georges Clemenceau, 14033 Caen, France.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Evaluation Studies
|