Source:http://linkedlifedata.com/resource/pubmed/id/15844357
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2005-4-22
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| pubmed:abstractText |
Disuccinimidyl suberate (DSS) intramolecularly cross-linked hemoglobin (Hb) was developed as a novel red blood cell substitute. A multi-angle laser light scattering detector coupled with size exclusion HPLC was applied to determine the molecular weight of the modified Hb. SDS-PAGE was also used as a complement. It was proved that 83.8% of the product was intramolecularly cross-linked Hb with weight-average molecular weights (Mw) of 67.5 kD, 12% was dimeric Hb with Mw of 146.6 kD, and 4.2% was trimeric Hb with Mw of 306.4 kD. The tetramer structure of the cross-linked Hb was stable as shown in size-exclusion chromatography using a mobile phase containing 1 mol/L MgCl2. Analysis by LC-MS demonstrated that the reaction of DSS with Hb mainly took place between the two alpha subunits within a Hb molecule, resulting in stabilization of the tetramer structure. However, the cross-linking was not site-specific. The P50 of the cross-linked Hb decreased from 21.8 mmHg to 14.3 mmHg, and the Hill coefficient decreased from 2.22 to 1.41. Result of isoelectric focusing showed that the pl of DSS cross-linked Hb was in the range of 4.6-5.2, similar to that of serum albumin. The safety of DSS cross-linked Hb was favored by animal tests on rats and guinea pigs. Exchange transfusion experiment with DSS cross-linked Hb using rats as a model indicated no pressor effect or other significant side effects. The characteristics and properties of DSS cross-linked Hb were also compared with that of diaspirin cross-linked Hb reported in the literature.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Substitutes,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides,
http://linkedlifedata.com/resource/pubmed/chemical/disuccinimidyl suberate
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1006-9305
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
48
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
49-60
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15844357-Animals,
pubmed-meshheading:15844357-Blood Substitutes,
pubmed-meshheading:15844357-Blood Transfusion,
pubmed-meshheading:15844357-Cattle,
pubmed-meshheading:15844357-Chromatography,
pubmed-meshheading:15844357-Chromatography, High Pressure Liquid,
pubmed-meshheading:15844357-Chromatography, Liquid,
pubmed-meshheading:15844357-Cross-Linking Reagents,
pubmed-meshheading:15844357-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15844357-Erythrocytes,
pubmed-meshheading:15844357-Guinea Pigs,
pubmed-meshheading:15844357-Hemoglobins,
pubmed-meshheading:15844357-Hydrogen-Ion Concentration,
pubmed-meshheading:15844357-Isoelectric Focusing,
pubmed-meshheading:15844357-Light,
pubmed-meshheading:15844357-Male,
pubmed-meshheading:15844357-Mass Spectrometry,
pubmed-meshheading:15844357-Models, Chemical,
pubmed-meshheading:15844357-Protein Conformation,
pubmed-meshheading:15844357-Rats,
pubmed-meshheading:15844357-Rats, Sprague-Dawley,
pubmed-meshheading:15844357-Scattering, Radiation,
pubmed-meshheading:15844357-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:15844357-Succinimides,
pubmed-meshheading:15844357-Time Factors
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| pubmed:year |
2005
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| pubmed:articleTitle |
Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute.
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| pubmed:affiliation |
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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