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pubmed:abstractText |
Bending of DNA is a feature essential to the function of many DNA-binding proteins. Bending angles can be estimated with a variety of techniques, but most directly from images obtained using scanning force microscopy (SFM). Direct measurement of the bending angle using a tangent method often produces angles that deviate significantly from values obtained using other techniques. Here, we describe the application of SFM in combination with simulations of DNA as a means to estimate protein-induced bending angles in a reliable and unbiased fashion. In this manner, we were able to obtain accurate estimates for the bending angles induced by nuclear factor I, octamer-binding transcription factor 1, the human XPC-Rad23B complex and integration host factor [correction]
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pubmed:affiliation |
Physics of Complex Systems, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit De Boelelaan 1081, NL-1081 HV, Amsterdam, The Netherlands. rtdame@nat.vu.nl
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