|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
2005-4-21
|
|
pubmed:abstractText |
Stimulation of the TCR leads to an oscillatory release of free calcium that activates members of the calcium/calmodulin-dependent protein kinase II (CaMKII) family. The CaMKII molecules have profound and lasting effects on cellular signaling in several cell types, yet the role of CaMKII in T cells is still poorly characterized. In this report we describe a splice variant of CaMKIIbeta, CaMKIIbeta'e, in mouse T cells. We have determined its function, along with that of CaMKIIgamma, by introducing the active and kinase-dead mutants into activated P14 TCR transgenic T cells using retroviral transduction. Active CaMKII enhanced the proliferation and cytotoxic activity of T cells while reducing their IL-2 production. Furthermore, it induced a profound state of unresponsiveness that could be overcome only by prolonged culture in IL-2. These results indicate that members of the CaMKII family play an important role in regulation of CD8 T cell proliferation, cytotoxic effector function, and the response to restimulation.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0022-1767
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
174
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5583-92
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15843557-Alternative Splicing,
pubmed-meshheading:15843557-Animals,
pubmed-meshheading:15843557-Antigens,
pubmed-meshheading:15843557-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15843557-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15843557-Calcium-Calmodulin-Dependent Protein Kinase Type 2,
pubmed-meshheading:15843557-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:15843557-Cell Proliferation,
pubmed-meshheading:15843557-Cell Survival,
pubmed-meshheading:15843557-Clonal Anergy,
pubmed-meshheading:15843557-Cytokines,
pubmed-meshheading:15843557-Cytotoxicity, Immunologic,
pubmed-meshheading:15843557-Enzyme Activation,
pubmed-meshheading:15843557-Isoenzymes,
pubmed-meshheading:15843557-Lymphocyte Activation,
pubmed-meshheading:15843557-Mice,
pubmed-meshheading:15843557-Mice, Inbred C57BL,
pubmed-meshheading:15843557-Mice, Transgenic,
pubmed-meshheading:15843557-Multienzyme Complexes,
pubmed-meshheading:15843557-Transduction, Genetic
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
A pivotal role for the multifunctional calcium/calmodulin-dependent protein kinase II in T cells: from activation to unresponsiveness.
|
|
pubmed:affiliation |
Division of Biological Sciences, The Cancer Center, and Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
