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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
2005-4-27
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pubmed:abstractText |
The calcium ion (Ca(2+)) is a ubiquitous second messenger that is crucial for the regulation of a wide variety of cellular processes. The diverse transient signals transduced by Ca(2+) are mediated by intracellular Ca(2+)-binding proteins, also known as Ca(2+) sensors. A key obstacle to studying many Ca(2+)-sensing proteins is the difficulty in identifying the numerous downstream target interactions that respond to Ca(2+)-induced conformational changes. Among a number of Ca(2+) sensors in the eukaryotic cell, calmodulin (CaM) is the most widespread and the best studied. Employing the mRNA display technique, we have scanned the human proteome for CaM-binding proteins and have identified and characterized a large number of both known and previously uncharacterized proteins that interact with CaM in a Ca(2+)-dependent manner. The interactions of several identified proteins with Ca(2+)/CaM were confirmed by using pull-down assays and coimmunoprecipitation. Many of the CaM-binding proteins identified belong to protein families such as the DEAD/H box proteins, ribosomal proteins, proteasome 26S subunits, and deubiquitinating enzymes, suggesting the possible involvement of Ca(2+)/CaM in different signaling pathways. The selection method described herein could be used to identify the binding partners of other calcium sensors on the proteome-wide scale.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-10077596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-10688190,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-10884684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-10889994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-10913138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-10982894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-11283018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-11370791,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-11782485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12052895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12589471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12618209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12633996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12821674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12836676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12838335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-12838338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-14605208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-14704431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-14991003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15242647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15247222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15294163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15358861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15499021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-1552844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15533439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-15544949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-1744106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-2186516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-2822712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-3754463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-7525583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-7663132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-7716515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-7834745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-7878743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-8226817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-8521483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-8848832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-9141499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-9356443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-9490418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-9601606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-9716384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15840729-9925766
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5969-74
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2005
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pubmed:articleTitle |
Scanning the human proteome for calmodulin-binding proteins.
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pubmed:affiliation |
Division of Medicinal Chemistry and Natural Products, School of Pharmacy, and Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, NC 27599, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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