Linked Life Data

15838989

Source:http://linkedlifedata.com/resource/pubmed/id/15838989

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-4-19
pubmed:abstractText
A single antibody-incubation step of an indirect, enzyme-linked immunosorbent assay (ELISA) was performed during microgravity, Martian gravity (0.38 G) and hypergravity (1.8 G) phases of parabolic flight, onboard the NASA KC-135 aircraft. Antibody-antigen binding occurred within 15 seconds; the level of binding did not differ between microgravity, Martian gravity and 1 G (Earth's gravity) conditions. During hypergravity and 1 G, antibody binding was directly proportional to the fluid volume (per microtiter well) used for incubation; this pattern was not observed during microgravity. These effects in microgravity may be due to "fluid spread" within the chamber (observed during microgravity with digital photography), leading to greater fluid-surface contact and subsequently antibody-antigen contact. In summary, these results demonstrate that: i) ELISA antibody-incubation and washing steps can be successfully performed by human operators during microgravity, Martian gravity and hypergravity; ii) there is no significant difference in antibody binding between microgravity, Martian gravity and 1 G conditions; and iii) a smaller fluid volume/well (and therefore less antibody) was required for a given level of binding during microgravity. These conclusions indicate that reduced gravity would not present a barrier to successful operation of immunosorbent assays during spaceflight.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1077-9248
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
47-55
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Antibody binding in altered gravity: implications for immunosorbent assay during space flight.
pubmed:affiliation
Geophysical Laboratory, Carnegie Institution of Washington, Washington, DC, USA. Jake.Maule@jsc.nasa.gov
pubmed:publicationType
Journal Article