Source:http://linkedlifedata.com/resource/pubmed/id/15838339
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2005-4-19
|
| pubmed:databankReference | |
| pubmed:abstractText |
To compare the structure of the precursor polypeptide of dog endothelin-3, preproendothelin-3 (PPET-3), with the PPET-3 of other mammals, we cloned dog cDNA from lung tissue using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. An open reading frame encoding a 198-amino-acid polypeptide was found in the cDNA. Regions corresponding to a bioactive mature endothelin-3 peptide, an intermediate form known as big-endothelin-3 and an endothelin-3- like peptide were observed in the putative PPET-3. Comparative analysis showed that the similarity of the dog open reading frame sequence with those from human hypothalamus, mouse intestine, and rat eye is 76.2%, 69.5% and 66.3%, respectively, and that the similarity at the amino acid level is 65.6%, 59.8% and 58.8%, respectively. RT-PCR demonstrated significant elevated expression of PPET-3 mRNA in the kidney of dog with interstitial nephritis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
|
| pubmed:issn |
1533-4023
|
| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
44 Suppl 1
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
S426-9
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| pubmed:meshHeading |
pubmed-meshheading:15838339-Amino Acid Sequence,
pubmed-meshheading:15838339-Animals,
pubmed-meshheading:15838339-Cloning, Molecular,
pubmed-meshheading:15838339-Disease Models, Animal,
pubmed-meshheading:15838339-Dogs,
pubmed-meshheading:15838339-Endothelin-3,
pubmed-meshheading:15838339-Humans,
pubmed-meshheading:15838339-Kidney,
pubmed-meshheading:15838339-Mice,
pubmed-meshheading:15838339-Molecular Sequence Data,
pubmed-meshheading:15838339-Nephritis, Interstitial,
pubmed-meshheading:15838339-Open Reading Frames,
pubmed-meshheading:15838339-Protein Precursors,
pubmed-meshheading:15838339-RNA, Messenger,
pubmed-meshheading:15838339-Rats,
pubmed-meshheading:15838339-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15838339-Sequence Alignment,
pubmed-meshheading:15838339-Sequence Analysis, Protein,
pubmed-meshheading:15838339-Sequence Homology, Amino Acid,
pubmed-meshheading:15838339-Up-Regulation
|
| pubmed:year |
2004
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| pubmed:articleTitle |
Primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.
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| pubmed:affiliation |
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|