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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-6-16
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pubmed:abstractText |
About 1.9% of ribosomes translating the gag open reading frame of the yeast L-A double-stranded RNA virus positive strand undergo a -1 frameshift and continue translating in the pol open reading frame to make a 170-kDa gag-pol fusion protein. The importance of frameshifting efficiency for viral propagation was tested in a system where the M1 (killer toxin-encoding) satellite RNA is supported by a full-length L-A cDNA clone. Either increasing or decreasing the frameshift efficiency more than twofold by alterations in the slippery site disrupted viral propagation. A threefold increase caused by a chromosomal mutation, hsh1 (high shifter), had the same effect. Substituting a +1 ribosomal frameshift site from Ty1 with the correct efficiency also allowed support of M1 propagation. The normal -1 frameshift efficiency is similar to the observed molar ratio in viral particles of the 170-kDa gag-pol protein to the 70-kDa gag gene product, the major coat protein. The results are interpreted in terms of a packaging model for L-A.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-1776357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-1870215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-1871115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-1880803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-1985195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-1986362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2005792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2117501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2164889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2168307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2193436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2199062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2247607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2388833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2416054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2452901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2460245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2651431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2720781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2763465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2823251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-2846182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3035577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3039147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3135981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3194229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3399386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3428275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3511153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3537705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-360211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-3889910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-6310321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-6371496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-6864790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-7002031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1583726-773743
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fusion Proteins, gag-pol,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/Killer Factors, Yeast,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Amino Acyl,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3669-76
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1583726-Base Sequence,
pubmed-meshheading:1583726-Frameshift Mutation,
pubmed-meshheading:1583726-Fungal Proteins,
pubmed-meshheading:1583726-Fusion Proteins, gag-pol,
pubmed-meshheading:1583726-Gene Products, gag,
pubmed-meshheading:1583726-Host-Parasite Interactions,
pubmed-meshheading:1583726-Killer Factors, Yeast,
pubmed-meshheading:1583726-Models, Genetic,
pubmed-meshheading:1583726-Molecular Sequence Data,
pubmed-meshheading:1583726-Mutation,
pubmed-meshheading:1583726-Nucleic Acid Conformation,
pubmed-meshheading:1583726-Proteins,
pubmed-meshheading:1583726-RNA, Double-Stranded,
pubmed-meshheading:1583726-RNA, Transfer, Amino Acyl,
pubmed-meshheading:1583726-RNA, Viral,
pubmed-meshheading:1583726-RNA Viruses,
pubmed-meshheading:1583726-Ribosomes,
pubmed-meshheading:1583726-Saccharomyces cerevisiae,
pubmed-meshheading:1583726-Virus Replication
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pubmed:year |
1992
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pubmed:articleTitle |
Ribosomal frameshifting efficiency and gag/gag-pol ratio are critical for yeast M1 double-stranded RNA virus propagation.
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pubmed:affiliation |
Section on the Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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