Source:http://linkedlifedata.com/resource/pubmed/id/15833849
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2005-4-18
|
| pubmed:abstractText |
Induction of neoangiogenesis plays an important role in the pathogenesis of multiple myeloma. However, the mechanism by which expression of vascular endothelial growth factor (VEGF)-A and its receptors modulate the interaction of multiple myeloma cells with stromal cells is not known. Here, we describe a novel in vitro coculture system using fetal bone stromal cells as a feeder layer, which facilitates the survival and growth of human primary multiple myeloma cells. We show that stromal-dependent paracrine VEGF-A signaling promotes proliferation of human primary multiple myeloma cells. Primary multiple myeloma cells only expressed functional VEGF receptor (VEGFR)-1, but not VEGFR-2 or VEGFR-3. VEGFR-1 expression was detected in the cytoplasm and the nuclei of proliferating multiple myeloma cells. Inhibition of VEGFR-1 abrogated multiple myeloma cell proliferation and motility, suggesting that the functional interaction of VEGF-A with its cognate receptor is essential for the growth of primary multiple myeloma cells. Collectively, our results suggest that stromal-dependent paracrine and intracrine VEGF-A/VEGFR-1 signaling contributes to human primary multiple myeloma cell growth and therefore, VEGFR-1 blockade is a potential therapeutic strategy for the treatment of multiple myeloma.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0008-5472
|
| pubmed:author |
pubmed-author:BohlenPeterP,
pubmed-author:HicklinDaniel JDJ,
pubmed-author:HooperAndrea TAT,
pubmed-author:JinDavid KDK,
pubmed-author:KarajannisMatthias AMA,
pubmed-author:NiesvizkyRubenR,
pubmed-author:PytowskiBronislawB,
pubmed-author:RíoLL,
pubmed-author:RafiiShahinS,
pubmed-author:RashbaumWilliam KWK,
pubmed-author:ShidoKojiK,
pubmed-author:VincentLoïcL,
pubmed-author:ZhuZhenpingZ
|
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3185-92
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:15833849-Bone and Bones,
pubmed-meshheading:15833849-Cell Growth Processes,
pubmed-meshheading:15833849-Cell Movement,
pubmed-meshheading:15833849-Cell Nucleus,
pubmed-meshheading:15833849-Cell Survival,
pubmed-meshheading:15833849-Coculture Techniques,
pubmed-meshheading:15833849-Cytoplasm,
pubmed-meshheading:15833849-Humans,
pubmed-meshheading:15833849-Multiple Myeloma,
pubmed-meshheading:15833849-Stromal Cells,
pubmed-meshheading:15833849-Tumor Cells, Cultured,
pubmed-meshheading:15833849-Vascular Endothelial Growth Factor A,
pubmed-meshheading:15833849-Vascular Endothelial Growth Factor Receptor-1
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Fetal stromal-dependent paracrine and intracrine vascular endothelial growth factor-a/vascular endothelial growth factor receptor-1 signaling promotes proliferation and motility of human primary myeloma cells.
|
| pubmed:affiliation |
Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA. vincentloicny@yahoo.com
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|