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pubmed-article:1583336pubmed:abstractTextThe polymerase chain reaction (PCR) was used to identify spores of Bacillus anthracis. By using an assay capable of amplifying a 1247-bp fragment from the gene that encodes the edema factor of B. anthracis, as few as 10(3) copies of a plasmid containing the edema factor gene and as few as 2 x 10(4) spores were detected. Subjecting the product of this PCR to a second PCR designed to amplify a 208-bp fragment nested within the 1247-bp product improved detection to a single plasmid copy per PCR and to two spores of B. anthracis per PCR.lld:pubmed
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pubmed-article:1583336pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1583336pubmed:articleTitleDetection of spores of Bacillus anthracis using the polymerase chain reaction.lld:pubmed
pubmed-article:1583336pubmed:affiliationNaval Medical Research Institute, Naval Medical Research and Development Command, Bethesda, Maryland.lld:pubmed
pubmed-article:1583336pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1583336pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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