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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1992-6-18
|
| pubmed:abstractText |
The polymerase chain reaction (PCR) was used to identify spores of Bacillus anthracis. By using an assay capable of amplifying a 1247-bp fragment from the gene that encodes the edema factor of B. anthracis, as few as 10(3) copies of a plasmid containing the edema factor gene and as few as 2 x 10(4) spores were detected. Subjecting the product of this PCR to a second PCR designed to amplify a 208-bp fragment nested within the 1247-bp product improved detection to a single plasmid copy per PCR and to two spores of B. anthracis per PCR.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1899
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
165
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1145-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1583336-Bacillus anthracis,
pubmed-meshheading:1583336-Base Sequence,
pubmed-meshheading:1583336-DNA, Bacterial,
pubmed-meshheading:1583336-Molecular Sequence Data,
pubmed-meshheading:1583336-Oligonucleotide Probes,
pubmed-meshheading:1583336-Polymerase Chain Reaction,
pubmed-meshheading:1583336-Sensitivity and Specificity,
pubmed-meshheading:1583336-Spores, Bacterial
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Detection of spores of Bacillus anthracis using the polymerase chain reaction.
|
| pubmed:affiliation |
Naval Medical Research Institute, Naval Medical Research and Development Command, Bethesda, Maryland.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|