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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-6-18
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pubmed:abstractText |
The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgG1 monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgG1, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxoid,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/cryptococcal polysaccharide
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1086-93
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1583327-Animals,
pubmed-meshheading:1583327-Antibodies, Fungal,
pubmed-meshheading:1583327-Antibodies, Monoclonal,
pubmed-meshheading:1583327-Antibody Specificity,
pubmed-meshheading:1583327-Antigens, Fungal,
pubmed-meshheading:1583327-Binding, Competitive,
pubmed-meshheading:1583327-Cryptococcosis,
pubmed-meshheading:1583327-Cryptococcus neoformans,
pubmed-meshheading:1583327-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1583327-Fungal Vaccines,
pubmed-meshheading:1583327-Hybridomas,
pubmed-meshheading:1583327-Immunoglobulin G,
pubmed-meshheading:1583327-Immunoglobulin M,
pubmed-meshheading:1583327-Mice,
pubmed-meshheading:1583327-Mice, Inbred BALB C,
pubmed-meshheading:1583327-Polysaccharides,
pubmed-meshheading:1583327-Tetanus Toxoid,
pubmed-meshheading:1583327-Vaccines, Synthetic
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pubmed:year |
1992
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pubmed:articleTitle |
Antibodies elicited by a Cryptococcus neoformans-tetanus toxoid conjugate vaccine have the same specificity as those elicited in infection.
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pubmed:affiliation |
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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