Linked Life Data

15832361

Source:http://linkedlifedata.com/resource/pubmed/id/15832361

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-6-9
pubmed:abstractText
Regulated splicing of fibronectin (FN) occurs during the mesenchymal to chondrocyte transition and ultimately results in the relative enrichment of an extra domain B (EDB) exon-containing FN isoform with the suggestion that FN isoforms may play a functional role in chondrogenesis. Promotion of chondrogenesis can also be achieved by treatment with transforming growth factor-beta (TGF-beta), which also regulates FN isoform expression. We have examined the effects of TGF-beta treatment on the assumption of the chondrogenic phenotype in the teratoma-derived cell line ATDC5 and tested whether these effects on chondrogenesis are paralleled by appropriate changes in FN isoform expression. ATDC5 cells were maintained in a pre-chondrogenic state and, in this state, treated with 10 ng/ml TGF-beta. The cells started to elaborate a matrix rich in sulfated proteoglycans, such that within the first 12 days of culture, TGF-beta1 treatment appeared to slightly accelerate early acquisition of an Alcian blue-stained matrix, and caused a dose- and time-dependent decrease in collagen type I expression; changes in collagen type II expression were variable. At later times, cells treated with TGF-beta became indistinguishable from those of the controls. Interestingly, TGF-beta treatment caused a significant dose- and time-dependent decrease in the proportion of FN containing the extra domain A (EDA) and the EDB exons. These data suggest that TGF-beta induces the early stages of chondrogenic maturation in this pre-chondrogenic line and that TGF-beta treatment increases expression of FN isoforms that lack the EDA and EDB exons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
750-62
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15832361-Alternative Splicing, pubmed-meshheading:15832361-Animals, pubmed-meshheading:15832361-Cell Differentiation, pubmed-meshheading:15832361-Cell Line, Tumor, pubmed-meshheading:15832361-Cell Proliferation, pubmed-meshheading:15832361-Chondrogenesis, pubmed-meshheading:15832361-Collagen Type I, pubmed-meshheading:15832361-Collagen Type II, pubmed-meshheading:15832361-Exons, pubmed-meshheading:15832361-Fibronectins, pubmed-meshheading:15832361-Gene Expression Regulation, pubmed-meshheading:15832361-Mice, pubmed-meshheading:15832361-Protein Isoforms, pubmed-meshheading:15832361-Proteoglycans, pubmed-meshheading:15832361-RNA, Messenger, pubmed-meshheading:15832361-Sulfates, pubmed-meshheading:15832361-Time Factors, pubmed-meshheading:15832361-Transforming Growth Factor beta, pubmed-meshheading:15832361-Transforming Growth Factor beta1
pubmed:year
2005
pubmed:articleTitle
Transforming growth factor-beta1 (TGF-beta1) regulates ATDC5 chondrogenic differentiation and fibronectin isoform expression.
pubmed:affiliation
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural