Linked Life Data

15831569

Source:http://linkedlifedata.com/resource/pubmed/id/15831569

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-6-10
pubmed:abstractText
Cocaine- and amphetamine-regulated transcript (CART) has stimulatory effects on the hypothalamic-pituitary-adrenal axis through direct effects on hypophysiotropic CRH neurons. Recently CART-containing axons have been demonstrated to densely innervate the hypophysiotropic CRH neurons. Based on the sources of the CART-immunoreactive (IR) innervation of the paraventricular nucleus, the putative origins of these CART-containing fibers include neurons of the hypothalamic arcuate nucleus that coexpress alphaMSH and medullary adrenaline-producing neurons. To determine whether these cell groups contribute to the CART innervation of the hypophysiotropic CRH neurons, we performed a quadruple-labeling immunofluorescent study using antisera against CRH, CART, alphaMSH, and phenylethanolamine-N-methyl-transferase (PNMT), the latter as a marker for adrenaline. Consistent with previous observations, PNMT- and CART-IR axons densely innervated all CRH neurons, whereas the alphaMSH-IR innervation was sparse. Although approximately 60% of CART-IR varicosities in juxtaposition to CRH neurons cocontained PNMT, only approximately 18% of them were immunopositive for alphaMSH. All alphaMSH-IR boutons and approximately 90% of PNMT-containing varicosities on the surface of CRH neurons were also labeled for CART. The remaining 22% of CART axon varicosities in contact with CRH neurons contained neither alphaMSH nor PNMT. These results indicate that medullary adrenergic/CART neurons are the major source for the CART innervation of CRH neurons in the paraventricular nucleus; however, to a lesser extent the arcuate nucleus also contributes to the CART-IR innervation of these neurons. The observation that nearly 20% of the CART-IR afferents contain neither alphaMSH nor PNMT, however, suggests that additional sources also contribute to the CART-IR input of hypophysiotropic CRH neurons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2985-91
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15831569-Animals, pubmed-meshheading:15831569-Arcuate Nucleus, pubmed-meshheading:15831569-Axons, pubmed-meshheading:15831569-Corticotropin-Releasing Hormone, pubmed-meshheading:15831569-Fluorescent Antibody Technique, pubmed-meshheading:15831569-Hypothalamo-Hypophyseal System, pubmed-meshheading:15831569-Male, pubmed-meshheading:15831569-Medulla Oblongata, pubmed-meshheading:15831569-Methyltransferases, pubmed-meshheading:15831569-Microscopy, Confocal, pubmed-meshheading:15831569-Nerve Tissue Proteins, pubmed-meshheading:15831569-Neurons, pubmed-meshheading:15831569-Neurotransmitter Agents, pubmed-meshheading:15831569-Phosphatidylethanolamine N-Methyltransferase, pubmed-meshheading:15831569-Pituitary-Adrenal System, pubmed-meshheading:15831569-Presynaptic Terminals, pubmed-meshheading:15831569-Rats, pubmed-meshheading:15831569-Rats, Wistar, pubmed-meshheading:15831569-Tissue Distribution, pubmed-meshheading:15831569-alpha-MSH
pubmed:year
2005
pubmed:articleTitle
Origin of cocaine- and amphetamine-regulated transcript-containing axons innervating hypophysiotropic corticotropin-releasing hormone-synthesizing neurons in the rat.
pubmed:affiliation
Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural