|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
2005-4-15
|
|
pubmed:abstractText |
We analyzed the impact of a GAGA element on a transgenic promoter in Drosophila melanogaster that was activated by proteins composed of the Tet(on) DNA binding domain and either the heat shock factor (HSF) activation domain or a potent subdomain of VP16. Permanganate footprinting was used to monitor polymerase II (Pol II) on the transgenic promoters in vivo. Activation by Tet(on)-HSF but not by Tet(on)-VP16(A2) required the GAGA element; this correlated with the ability of the GAGA element to establish a paused Pol II. Although the GAGA element was not required for activation by Tet(on)-VP16(A2), the GAGA element greatly accelerated the rate of activation. The permanganate data also provided evidence that Pol II encountered different rate-limiting steps, following initiation in the presence of Tet(on)-HSF and Tet(on)-VP16(A2). The rate-limiting step in the presence of Tet(on)-HSF was release of Pol II paused about 20 to 40 nucleotides downstream from the start site. The rate-limiting step in the presence of Tet(on)-VP16(A2) occurred much closer to the transcription start site. Several biochemical studies have provided evidence for a structural transition shortly after Pol II initiates transcription. The behavior of Pol II in the presence of Tet(on)-VP16(A2) provides the first evidence that this transition occurs in vivo.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-10384299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-10713179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-10859354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-10912001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-11511356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-11583616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-11784853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-12167709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-12192035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-12213653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-12601174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-12782658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1310361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1319065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1377279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1427079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1427080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-15053881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-15479635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-15741180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1737619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-1922045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-2257625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-2722774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-2781290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-3246353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-3667620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-7068686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-7590251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-7739554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-7744251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-7838531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-8107823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-8367444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-8521501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-8628664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-8816456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9199313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9207017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9321643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9405375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9407112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9671550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15831460-9927429
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0270-7306
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
25
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3543-52
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:15831460-Animals,
pubmed-meshheading:15831460-DNA Footprinting,
pubmed-meshheading:15831460-DNA-Binding Proteins,
pubmed-meshheading:15831460-Drosophila melanogaster,
pubmed-meshheading:15831460-HSP70 Heat-Shock Proteins,
pubmed-meshheading:15831460-Heat-Shock Proteins,
pubmed-meshheading:15831460-Herpes Simplex Virus Protein Vmw65,
pubmed-meshheading:15831460-Protein Structure, Tertiary,
pubmed-meshheading:15831460-RNA Polymerase II,
pubmed-meshheading:15831460-Recombinant Fusion Proteins,
pubmed-meshheading:15831460-Response Elements,
pubmed-meshheading:15831460-Trans-Activators,
pubmed-meshheading:15831460-Transcription Initiation Site,
pubmed-meshheading:15831460-Transcriptional Activation,
pubmed-meshheading:15831460-Transgenes
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Identification in vivo of different rate-limiting steps associated with transcriptional activators in the presence and absence of a GAGA element.
|
|
pubmed:affiliation |
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
|
