| pubmed-article:15830130 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15830130 | lifeskim:mentions | umls-concept:C0230463 | lld:lifeskim |
| pubmed-article:15830130 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:15830130 | lifeskim:mentions | umls-concept:C0080347 | lld:lifeskim |
| pubmed-article:15830130 | lifeskim:mentions | umls-concept:C0040648 | lld:lifeskim |
| pubmed-article:15830130 | lifeskim:mentions | umls-concept:C0017263 | lld:lifeskim |
| pubmed-article:15830130 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15830130 | pubmed:dateCreated | 2005-4-14 | lld:pubmed |
| pubmed-article:15830130 | pubmed:abstractText | The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DNA recognition domains such that the ZFP TF binds a contiguous DNA target site - thus emulating the design of ZFP TFs described previously for mammalian gene regulation. We show that this plant-based ZFP TF architecture supports high affinity DNA binding while allowing the specificity of the DNA-protein interaction to be determined by the amino acid sequences of the recognition helices. This plant-based backbone thus supports the use of previously characterized DNA recognition helices originally identified in a mammalian ZFP context without using mammalian DNA sequences. Moreover, we show that plant-based ZFP TFs employing this new architecture can up-regulate endogenous ADH activity by > 20-fold in transgenic Arabidopsis. Thus plant-based ZFP TFs are shown to be potent regulators of gene expression in vivo. | lld:pubmed |
| pubmed-article:15830130 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15830130 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15830130 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15830130 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15830130 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:15830130 | pubmed:issn | 0167-4412 | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:LiuQiangQ | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:CaseCasey CCC | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:RebarEdward... | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:LiGuofuG | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:Holmes-DavisR... | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:GregoryPhilip... | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:JamiesonAndre... | lld:pubmed |
| pubmed-article:15830130 | pubmed:author | pubmed-author:KongYanhongY | lld:pubmed |
| pubmed-article:15830130 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15830130 | pubmed:volume | 57 | lld:pubmed |
| pubmed-article:15830130 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15830130 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15830130 | pubmed:pagination | 411-23 | lld:pubmed |
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| pubmed-article:15830130 | pubmed:meshHeading | pubmed-meshheading:15830130... | lld:pubmed |
| pubmed-article:15830130 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15830130 | pubmed:articleTitle | Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors. | lld:pubmed |
| pubmed-article:15830130 | pubmed:affiliation | Plant Gene Expression Center, USDA, Albany, CA 94710, USA. | lld:pubmed |
| pubmed-article:15830130 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15830130 | lld:pubmed |
