|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2005-4-14
|
|
pubmed:abstractText |
The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DNA recognition domains such that the ZFP TF binds a contiguous DNA target site - thus emulating the design of ZFP TFs described previously for mammalian gene regulation. We show that this plant-based ZFP TF architecture supports high affinity DNA binding while allowing the specificity of the DNA-protein interaction to be determined by the amino acid sequences of the recognition helices. This plant-based backbone thus supports the use of previously characterized DNA recognition helices originally identified in a mammalian ZFP context without using mammalian DNA sequences. Moreover, we show that plant-based ZFP TFs employing this new architecture can up-regulate endogenous ADH activity by > 20-fold in transgenic Arabidopsis. Thus plant-based ZFP TFs are shown to be potent regulators of gene expression in vivo.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0167-4412
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
57
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
411-23
|
|
pubmed:meshHeading |
pubmed-meshheading:15830130-Alcohol Dehydrogenase,
pubmed-meshheading:15830130-Amino Acid Sequence,
pubmed-meshheading:15830130-Arabidopsis,
pubmed-meshheading:15830130-Binding, Competitive,
pubmed-meshheading:15830130-Binding Sites,
pubmed-meshheading:15830130-DNA-Binding Proteins,
pubmed-meshheading:15830130-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:15830130-Gene Expression Regulation, Plant,
pubmed-meshheading:15830130-Molecular Sequence Data,
pubmed-meshheading:15830130-Plant Leaves,
pubmed-meshheading:15830130-Plant Proteins,
pubmed-meshheading:15830130-Plants, Genetically Modified,
pubmed-meshheading:15830130-RNA, Messenger,
pubmed-meshheading:15830130-Recombinant Fusion Proteins,
pubmed-meshheading:15830130-Sequence Homology, Amino Acid,
pubmed-meshheading:15830130-Transcription Factors,
pubmed-meshheading:15830130-Up-Regulation,
pubmed-meshheading:15830130-Zinc Fingers
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors.
|
|
pubmed:affiliation |
Plant Gene Expression Center, USDA, Albany, CA 94710, USA.
|
|
pubmed:publicationType |
Journal Article
|
