Linked Life Data

15829635

Source:http://linkedlifedata.com/resource/pubmed/id/15829635

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2005-4-14
pubmed:abstractText
Active zones are presynaptic regions where synaptic vesicles fuse with plasma membrane to release neurotransmitters. Active zones are highly organized structurally and are functionally conserved among different species. Synapse defective-2 (SYD-2) family proteins regulate active zone morphology in Caenorhabditis elegans and Drosophila. Here, we demonstrate by immunoelectron microscopy that at C. elegans synapses, SYD-2 localizes strictly at active zones and can be used as an active zone marker when fused to green fluorescent protein (GFP). By driving expression of SYD-2::GFP fusion protein in GABAergic neurons, we are able to visualize discrete fluorescent puncta corresponding to active zones in living C. elegans. During development, the number of GABAergic synapses made by specific motoneurons increases only slightly from larvae to adult stages. In contrast, the number of SYD-2::GFP puncta doubles, suggesting that individual synapses accommodate the increasing size of their synaptic targets mainly by incorporating more active zone materials. Furthermore, we used this marker to perform a genetic screen to identify genes involved in the development of active zones. We recovered 16 mutants with altered SYD-2::GFP expression, including alleles of five genes that have been implicated previously in synapse formation or nervous-system development. Mapping of 11 additional mutants suggests that they may represent novel genes involved in active zone formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
13
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3833-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15829635-Animals, pubmed-meshheading:15829635-Biological Markers, pubmed-meshheading:15829635-Caenorhabditis elegans, pubmed-meshheading:15829635-Caenorhabditis elegans Proteins, pubmed-meshheading:15829635-Carrier Proteins, pubmed-meshheading:15829635-Cell Membrane, pubmed-meshheading:15829635-Gene Expression Regulation, Developmental, pubmed-meshheading:15829635-Green Fluorescent Proteins, pubmed-meshheading:15829635-Immunohistochemistry, pubmed-meshheading:15829635-Microscopy, Immunoelectron, pubmed-meshheading:15829635-Molecular Sequence Data, pubmed-meshheading:15829635-Organogenesis, pubmed-meshheading:15829635-Phosphoproteins, pubmed-meshheading:15829635-Presynaptic Terminals, pubmed-meshheading:15829635-Synapses, pubmed-meshheading:15829635-Synaptic Vesicles, pubmed-meshheading:15829635-gamma-Aminobutyric Acid
pubmed:year
2005
pubmed:articleTitle
Identification of genes involved in synaptogenesis using a fluorescent active zone marker in Caenorhabditis elegans.
pubmed:affiliation
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't