Linked Life Data

15829633

Source:http://linkedlifedata.com/resource/pubmed/id/15829633

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2005-4-14
pubmed:abstractText
Receptor-type protein tyrosine phosphatases (RPTPs) are required for appropriate growth of axons during nervous system development in Drosophila. In the vertebrate, type IIa RPTPs [protein tyrosine phosphatase (PTP)-delta, PTP-sigma, and LAR (leukocyte common-antigen-related)] and the type III RPTP, PTP receptor type O (PTPRO), have been implicated in the regulation of axon growth, but their roles in developmental axon guidance are unclear. PTPRO, PTP-delta, and PTP-sigma are each expressed in chick motor neurons during the period of axonogenesis. To examine potential roles of RPTPs in axon growth and guidance in vivo, we used double-stranded RNA (dsRNA) interference combined with in ovo electroporation to knock down RPTP expression levels in the embryonic chick lumbar spinal cord. Although most branches of the developing limb nerves appeared grossly normal, a dorsal nerve identified as the anterior iliotibialis was clearly affected by dsRNA knock-down of RPTPs. In experimental embryos treated with dsRNA targeting PTP-delta, PTP-sigma, or PTPRO, this nerve showed abnormal fasciculation, was reduced in size, or was missing entirely; interference with PTPRO produced the most severe phenotypes. Control embryos electroporated with vehicle, or with dsRNA targeting choline acetyltransferase or axonin-1, did not exhibit this phenotype. Surprisingly, embryos electroporated with dsRNA targeting PTP-delta together with PTPRO, or all three RPTPs combined, had less severe phenotypes than embryos treated with PTPRO alone. This result suggests that competition between type IIa and type III RPTPs can regulate motor axon outgrowth, consistent with findings in Drosophila. Our results indicate that RPTPs, and especially PTPRO, are required for axon growth and guidance in the developing vertebrate limb.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
13
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3813-23
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15829633-Amino Acids, pubmed-meshheading:15829633-Animals, pubmed-meshheading:15829633-Avian Proteins, pubmed-meshheading:15829633-Axons, pubmed-meshheading:15829633-Blotting, Western, pubmed-meshheading:15829633-Cell Adhesion Molecules, Neuronal, pubmed-meshheading:15829633-Chick Embryo, pubmed-meshheading:15829633-Contactin 2, pubmed-meshheading:15829633-Drosophila, pubmed-meshheading:15829633-Electroporation, pubmed-meshheading:15829633-Embryo, Nonmammalian, pubmed-meshheading:15829633-Gene Expression Regulation, Developmental, pubmed-meshheading:15829633-Green Fluorescent Proteins, pubmed-meshheading:15829633-In Situ Hybridization, pubmed-meshheading:15829633-Motor Neurons, pubmed-meshheading:15829633-Phenotype, pubmed-meshheading:15829633-Protein Tyrosine Phosphatases, pubmed-meshheading:15829633-RNA, Double-Stranded, pubmed-meshheading:15829633-RNA, Messenger, pubmed-meshheading:15829633-Receptors, Cell Surface, pubmed-meshheading:15829633-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15829633-Spinal Cord, pubmed-meshheading:15829633-Xenopus Proteins
pubmed:year
2005
pubmed:articleTitle
Receptor tyrosine phosphatases guide vertebrate motor axons during development.
pubmed:affiliation
Neuroscience Program, Miami Project to Cure Paralysis, University of Miami School of Medicine, Miami, Florida 33136, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural