Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-6-15
pubmed:abstractText
Jejunal biopsies from 16 treated coeliac disease patients and from nine controls were cultured with and without a peptic-tryptic digest of gliadin. Cultures with a peptic-tryptic digest of maize prolamins were also undertaken. Frozen sections of baseline and cultured mucosa were stained by immunofluorescence with an anti-HLA-DR monoclonal antibody. Before culture the villous epithelium from both controls and treated coeliac disease expressed DR molecules while the crypt epithelium did not. When biopsies from treated coeliac disease were cultured with gliadin the expression of DR was enhanced in the crypt epithelium in eight of 14 cultures and in 11 of 14 was reduced or absent on the villous epithelium. No change was observed in control cultures. We conclude that gliadin is capable of inducing HLA-DR on the crypt epithelium of in vitro cultured coeliac disease mucosa, providing indirect evidence that gliadin may activate cell mediated immune mechanisms within the small bowel mucosa. This model could prove useful in identifying the immunogenic sequence(s) of gliadins and related prolamins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-1091964, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-11344562, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-1902808, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-2423435, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-2960770, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3040543, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3146453, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3151441, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3261663, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3277130, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3308219, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3311907, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3322617, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3335296, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3500504, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3514010, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-3758613, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-5157137, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-6425398, http://linkedlifedata.com/resource/pubmed/commentcorrection/1582589-7155669
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0017-5749
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
472-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Gliadin induced changes in the expression of MHC-class II antigens by human small intestinal epithelium. Organ culture studies with coeliac disease mucosa.
pubmed:affiliation
Cattedra di Gastroenterologia, Università di Roma, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't